Ethylene-responsive factor HvERF72 regulates starch synthesis and B-type starch granules initiation in barley.

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-11-15 Epub Date: 2025-08-05 DOI:10.1016/j.carbpol.2025.124167

Gaozong Zhou, Jinjin Ding, Yulong Li, Qiang Xu, Yazhou Zhang, Huaping Tang, Pengfei Qi, Mei Deng, Jian Ma, Guoyue Chen, Jirui Wang, Na Lin, Yuming Wei, Qiantao Jiang

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引用次数: 0

Abstract

Starch biosynthesis is a pivotal determinant of barley grain quality and yield, yet its regulatory mechanisms remain incompletely characterized. This study identifies HvERF72, an AP2-domain transcription factor, as a key regulator of starch biosynthesis and granule initiation in barley grains. Comparative analyses of CRISPR/Cas9-generated HvERF72 knockout mutants revealed enhanced B-type granule formation and elevated total starch content, whereas overexpression lines exhibited contrasting phenotypes, including reduced starch accumulation and suppressed B-type granule initiation. Transcriptional profiling at 15 DAF indicated significant upregulation of critical starch biosynthesis genes (HvAGPL1, HvAGPS1, HvSS2a, HvSBEI, HvSBEIIb, and HvGBSSI) in mutants, while overexpression lines showed downregulation of these genes. Mechanistic investigation demonstrated that HvERF72 directly binds to GCC-box motifs in the promoter regions of HvSS2a and HvSBEI, repressing their transcription. These findings establish HvERF72 as dual-function regulator that modulates starch biosynthesis and B-type granule initiation, providing novel molecular targets for optimizing starch yield and industrial quality in barley breeding programs.

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乙烯响应因子HvERF72调控大麦淀粉合成和b型淀粉粒形成。

淀粉生物合成是大麦籽粒品质和产量的关键决定因素，但其调控机制尚不完全清楚。本研究发现ap2结构域转录因子HvERF72是大麦籽粒淀粉生物合成和颗粒形成的关键调控因子。CRISPR/ cas9产生的HvERF72敲除突变体的比较分析显示，b型颗粒形成增强，总淀粉含量升高，而过表达系表现出相反的表型，包括淀粉积累减少和b型颗粒形成抑制。15 DAF的转录谱分析显示，突变体中关键淀粉生物合成基因（HvAGPL1、HvAGPS1、HvSS2a、HvSBEI、HvSBEIIb和HvGBSSI）显著上调，而过表达系显示这些基因下调。机制研究表明，HvERF72直接结合HvSS2a和HvSBEI启动子区域的GCC-box基序，抑制其转录。这些发现证实了HvERF72是调节淀粉生物合成和b型颗粒起始的双功能调节剂，为优化大麦育种计划中的淀粉产量和工业品质提供了新的分子靶点。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.