Concentration-dependent responses of C. reinhardtii to silver ions: hormetic response in growth and reduction of motility

Abstract

Elevated levels of silver in aquatic environments arising from widespread use of silver nitrate and silver nanoparticles in different sectors of industry and medicine pose significant biophysical challenges to aquatic microorganisms. Despite extensive toxicity studies of silver on bacteria and microbial communities, its influence on other aquatic microorganisms, such as microalgae, remains poorly understood. In this study, we investigated the biophysical response of C. reinhardtii microalgae to silver ion exposure in terms of their population growth dynamics, chlorophyll content, and swimming motility. We found that silver ions at different concentrations (from 0.29 to 1.18 \(\upmu \)M) elongated the lag phase of the microalgal growth. However, the growth of the microalgae was boosted by silver ions at low concentrations (e.g., 0.29 \(\upmu \)M), showing higher OD₇₅₀ values at the stationary phase and higher maximum growth rates. This hormetic response exhibited by microalgae upon exposure to silver ions indicates a nonlinear coupling between ionic stress and cellular growth. Additionally, we quantified the chlorophyll content in the microalgae with different concentrations of silver ions using spectrophotometric analysis, which revealed that the microalgae cells contained twice as high concentrations of chlorophyll when exposed to silver ions at lower concentrations. More importantly, we monitored the motion of microalgae in the presence of silver ions, detected and tracked their motion using a deep learning algorithm, and determined the effects of silver ions on the swimming motility of individual C. reinhardtii microalgae. Our results showed reduced average swimming speed and increased directional change of microalgae upon silver ion exposure.