The influence of water flow disturbance form on the granulation of algae sludge: Granulation property, treatment effect and algae biological structure

IF 6.7 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-09-15 DOI:10.1016/j.jwpe.2025.108691

Peng-fei Yu, Ao Wang, Hai-wen Sun, Ding Wang, Xing-guan Ma, Yu-jia Wang, Bo-mei Hu, Li-yao Han, Yu-bo Fu, Dian-lin Jiang, Shi-gang Tu

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Abstract

Algal-bacterial granular sludge (ABGS) is a research hotspot in sewage treatment. Water flow disturbance is crucial for sludge granulation in the algae-bacteria system, yet the effects of different disturbance modes on ABGS formation remain unclear. Horizontal stirring and supernatant reflux were used to start the ABGS system, the results showed the mode of horizontal disturbance combined with aeration is more conducive to the enrichment of algae and the formation of granular faster(80 day granulation was essentially completed), and the granular size is larger(more than 1.5 mm), high biomass (mixed liquid suspended solids for 8180 ± 183.61 mg/L) and good settleability (sludge volume index for 25.97 ± 3.00 mL/g), the extracellular polymeric substances secretion is more(147.32 ± 2.30 mg/g VSS), and the removal of nitrogen (N) and phosphorus (P) by horizontal stirring mode is higher, reaching 95.64 % and 99.58 %. High-throughput analyses revealed that horizontal agitation is more conducive to the proliferation of Nitrospira, Flavobacterium and Halochlorella, Scenedesmus taxa associated with nitrogen and phosphorus removal. Kyoto Encyclopedia of Genes and Genomes analysis demonstrated that in the quorum sensing system of the horizontal stirring method, the (suppressor of cell division inhibitor) gene regulated Tricarboxylic Acid cycle and N, P metabolism-related genes (ammonia monooxygenase, nitrite reductase 6-pyrrolidine tetrahydrobiopterin synthase), thereby enhancing metabolic gene expression. Supernatant reflux increased the expression of Uridine Diphosphate genes associated with polysaccharide production.

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水流扰动形式对藻类污泥制粒的影响：制粒特性、处理效果及藻类生物结构

藻菌颗粒污泥（ABGS）是污水处理领域的研究热点。在藻-菌系统中，水流扰动对污泥颗粒化至关重要，但不同扰动模式对污泥颗粒化的影响尚不清楚。采用水平搅拌和上清回流启动ABGS系统，结果表明，水平扰动结合曝气的模式更有利于藻类的富集和颗粒的形成速度更快（80天造粒基本完成），且颗粒尺寸较大（大于1.5 mm），生物量高（混合液悬浮固体为8180±183.61 mg/L），沉降性好（污泥体积指数为25.97±3.00 mL/g）。胞外聚合物质的分泌量更高（147.32±2.30 mg/g VSS），水平搅拌方式对氮(N)和磷(P)的去除率更高，分别达到95.64%和99.58%。高通量分析表明，水平搅拌更有利于硝化螺旋菌、黄杆菌和Halochlorella、Scenedesmus等与氮磷去除有关的分类群的增殖。京都基因与基因组百科全书分析表明，在水平搅拌法的群体感应系统中，（抑制细胞分裂抑制剂）基因调控三羧酸循环和N、P代谢相关基因（氨单加氧酶、亚硝酸盐还原酶6-吡啶四氢生物蝶呤合酶），从而增强代谢基因的表达。上清回流增加了与多糖产生相关的尿苷二磷酸基因的表达。

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来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies