Oral nutraceutical intervention to improve endothelial function: Evidence from in vitro and pilot clinical studies

IF 7.5 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2025-09-15 DOI:10.1016/j.biopha.2025.118552

Vincenza Valerio , Veronika A. Myasoedova , Ilaria Massaiu , Alessio Ravani , Beatrice Frigerio , Valentina Rusconi , Francesca Bertolini , Giulio Pompilio , Paolo Poggio

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引用次数: 0

Abstract

Objective

Aortic valve sclerosis affects 30 % of individuals over 65 and is associated with coronary artery disease, with risk of progression to aortic stenosis. Endothelial dysfunction, mediated by oxidative stress, impaired nitric oxide (NO) signaling, inflammation, and lipoprotein deposition, plays a central role in disease initiation and progression. This study investigated whether a combination of bioactive compounds could counteract these mechanisms and support vascular health.

Methods

The effects of curcuma longa, coenzyme Q10, black garlic, vitamin B1, and vitamin D3 were tested in vitro on aortic valve endothelial cells. Cell viability, reactive oxygen species (ROS), and NO levels were quantified by commercially available kits, while gene expression was analyzed by RNA sequencing. A 4-week prospective pilot clinical study in 10 healthy volunteers without cardiovascular disease evaluated endothelial function and arterial stiffness.

Results

The compounds reduced ROS production (>27 %; p < 0.05), enhanced endothelial viability (>33 %; p < 0.05; except curcuma and black garlic), and increased NO production (>6 %; p < 0.05; except black garlic). Beneficial effects were reflected in upregulation of anti-atherosclerotic (GIPR, +0.058 copies per million, CPM; p < 0.05), antioxidant (GADL1, +0.55 CPM; p < 0.001), and anti-inflammatory (IL12A, +0.17 CPM; p < 0.01) genes. Clinically, daily supplementation improved endothelial function in participants found to have pre-existing endothelial dysfunction (p = 0.0336), with 50 % achieving normal levels after 4 weeks, while all subjects exhibited reduced arterial stiffness (p = 0.0016) without hepatic toxicity.

Conclusions

The oral supplementation of the combination of these bioactive compounds improved endothelial function and vascular health, particularly in individuals with endothelial dysfunction, offering potential therapeutic benefits for cardiovascular health.

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口服营养品干预改善内皮功能：来自体外和初步临床研究的证据

目的：主动脉瓣硬化影响30 %的65岁以上人群，并与冠状动脉疾病相关，有进展为主动脉狭窄的风险。由氧化应激、一氧化氮（NO）信号受损、炎症和脂蛋白沉积介导的内皮功能障碍在疾病的发生和发展中起着核心作用。这项研究调查了生物活性化合物的组合是否可以抵消这些机制并支持血管健康。方法采用体外实验方法，观察姜黄、辅酶Q10、黑蒜、维生素B1、维生素D3对主动脉瓣内皮细胞的影响。通过市售试剂盒定量细胞活力、活性氧（ROS）和NO水平，同时通过RNA测序分析基因表达。一项在10名无心血管疾病的健康志愿者中进行的为期4周的前瞻性先导临床研究评估了内皮功能和动脉硬度。ResultsThe化合物减少ROS生产(在27 %;p & lt; 0.05),增强内皮可行性(祝辞33 %;p & lt; 0.05;除了姜黄和黑蒜),并增加生产(在6 %;p & lt; 0.05;黑蒜除外)。有益作用反映在抗动脉粥样硬化（GIPR, +0.058拷贝/百万，CPM; p <； 0.05），抗氧化（GADL1, +0.55 CPM; p <； 0.001）和抗炎（IL12A, +0.17 CPM; p <； 0.01）基因的上调上。在临床上，每天补充叶酸可改善存在内皮功能障碍的受试者的内皮功能（p = 0.0336），4周后50% %达到正常水平，而所有受试者均表现出动脉僵硬度降低（p = 0.0016），且无肝毒性。结论口服补充这些生物活性化合物可改善内皮功能和血管健康，特别是对内皮功能障碍患者，为心血管健康提供潜在的治疗益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.