CoWRKY53 positively modulates low phosphate tolerance through facilitation of CoPHO1;H3 in Camellia oleifera

IF 6.2 1区 农林科学 Q1 HORTICULTURE
Juanjuan Chen, Xiaojiao Han, Yuchen Yang, Linxiu Liu, Maolin Chai, Renying Zhuo, Xiaohua Yao
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引用次数: 0

Abstract

The responses of plants to abiotic stress have been widely investigated using WRKY transcription factors. Low environmental phosphate (Pi) levels typically result in reductions in both plant yield and quality. Currently, limited reports have been published on the response of WRKY transcription factors to low Pi exposure among woody plants that grow within the acidic soils of mountains. Herein, the CoWRKY53 gene in Camellia oleifera was recognized, and its relevance under low Pi conditions was explored. The CoWRKY53 gene was highly induced in root under low Pi stress. Specifically, CoWRKY53 is a nuclear protein through tobacco subcellular localization experiment, and it functions as a transcriptional activator. Moreover, it was demonstrated that overexpression of CoWRKY53 in Arabidopsis and poplars enhanced the biomass, acid phosphatase activity, and Pi concentration of these plants by altering the architecture of their root systems (RSA). Additionally, CoWRKY53 is directly associated with the W-box element of the CoPHO1;H3 promoter by yeast hybridization to promote its transcription in order to positively modulate low Pi tolerance. CoPHO1;H3 is known to complement Arabidopsis mutants. Thus, overexpression of CoPHO1;H3 in Arabidopsis also promoted resistance to low Pi exposure. Together, these results suggest that CoWRKY53 positively regulates CoPHO1;H3 to increase low Pi tolerance in plants. These results provide an important theoretical basis for further breeding of elite germplasm resources with strong low phosphate tolerance.
CoWRKY53通过促进CoPHO1正向调节低磷酸盐耐受性;油茶中的H3
利用WRKY转录因子对植物对非生物胁迫的响应进行了广泛的研究。低环境磷酸盐(Pi)水平通常会导致植物产量和质量的降低。目前,关于生长在山区酸性土壤中的木本植物WRKY转录因子对低磷暴露的响应报道有限。本文识别了油茶中的CoWRKY53基因,并探讨了其在低Pi条件下的相关性。低磷胁迫下,CoWRKY53基因在根中被高度诱导。具体来说,通过烟草亚细胞定位实验,CoWRKY53是一个核蛋白,其功能是转录激活因子。此外,CoWRKY53在拟南芥和杨树中的过表达通过改变根系结构(RSA)提高了这些植物的生物量、酸性磷酸酶活性和Pi浓度。此外,CoWRKY53与CoPHO1的W-box元素直接相关;H3启动子通过酵母杂交促进其转录,从而正向调节低π耐受性。CoPHO1;已知H3可以补充拟南芥突变体。因此,CoPHO1过表达;H3在拟南芥中也促进了对低π暴露的抗性。综上所述,这些结果表明CoWRKY53正调控CoPHO1;H3增加植物对低π的耐受性。这些结果为进一步选育耐低磷优良种质资源提供了重要的理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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