PbrMYB31–PbrbHLH147 complex activity in response to ABA promotes fatty acid biosynthesis for suberin deposition in the russet mutant of ‘Dangshansuli’

IF 6.2 1区 农林科学 Q1 HORTICULTURE
Xiexuan Wang, Lingxia Chen, Lindong Dou, Jinhui Yu, Xueqian Wang, Satoru Kondo, Takanori Saito, ZiXiao Wu, Jie Denga, Bing Jia, Zhenfeng Ye, Lun Liu, Xiaomei Tang, Li Liu, Wei Heng
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引用次数: 0

Abstract

Suberin is a naturally occurring polymer found in plant cell walls that plays crucial roles in protecting plants from environmental stress and enhancing resistance to pathogens. Despite its biological importance, the molecular regulatory networks governing suberin biosynthesis are not fully understood. In the russet pericarp of ‘Dangshanjinsu’ pear, suberin accumulation, which is enriched with very long-chain fatty acids, contributes to the formation of a robust protective barrier. Transcriptome data analysis revealed that PbrMYB31 and PbrbHLH147 were the key differentially expressed transcription factors in the pericarp of both ‘Dangshansuli’ and ‘Dangshanjinsu’ at various developmental stages. In this study, overexpression of PbrMYB31 and PbrbHLH147 was found to positively regulate long-chain fatty acid synthesis, promote suberin formation, and increase sensitivity to abscisic acid (ABA). Additionally, PbrMYB31 interacts with PbrbHLH147, forming a regulatory complex that synergistically extends the carbon chain length of fatty acids, a critical step in suberin assembly. ABA signaling was shown to integrate into this pathway, further modulating suberin production and linking hormonal regulation with structural barrier formation. This study provides novel insights into the genetic and hormonal control of suberin biosynthesis in russeted pear pericarp, emphasizing its critical role in enhancing fruit protection, improving quality, and extending shelf life, thereby contributing to advancements in horticultural science and sustainable fruit production.
‘砀山苏里’赤褐色突变体PbrMYB31-PbrbHLH147复合物活性对ABA的响应促进了脂肪酸的生物合成和沉积
木质素是一种天然存在于植物细胞壁中的聚合物,在保护植物免受环境胁迫和增强对病原体的抵抗力方面起着至关重要的作用。尽管其具有重要的生物学意义,但控制亚木质素生物合成的分子调控网络尚未完全了解。砀山金苏梨赤褐色果皮中富含极长链脂肪酸的木素积累有助于形成坚固的保护屏障。转录组数据分析显示,PbrMYB31和PbrbHLH147是党山苏里和党山金苏在不同发育阶段果皮中差异表达的关键转录因子。本研究发现,PbrMYB31和PbrbHLH147过表达可正向调节长链脂肪酸的合成,促进亚木素的形成,并增加对脱落酸(ABA)的敏感性。此外,PbrMYB31与PbrbHLH147相互作用,形成一个调节复合物,协同延长脂肪酸的碳链长度,这是亚木质素组装的关键步骤。ABA信号被证明整合到这一途径中,进一步调节亚木质素的产生,并将激素调节与结构屏障的形成联系起来。本研究为红梨果皮中木参素生物合成的遗传和激素调控提供了新的见解,强调了木参素在加强果实保护、提高品质和延长保质期方面的重要作用,从而为园艺科学和可持续水果生产的进步做出贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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