Pantinin-Derived Peptides against Veterinary Herpesviruses: Activity and Structural Characterization.

IF 3.4 4区 医学 Q2 CHEMISTRY, MEDICINAL
ChemMedChem Pub Date : 2025-09-14 DOI:10.1002/cmdc.202500333
Rosa Giugliano, Carla Zannella, Annalisa Chianese, Clementina Acconcia, Alessandra Monti, Roberta Della Marca, Ugo Pagnini, Serena Montagnaro, Nunzianna Doti, Carla Isernia, Massimiliano Galdiero, Filomena Fiorito, Luigi Russo, Valentina Iovane, Anna De Filippis
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引用次数: 0

Abstract

Animal viral infections represent a growing public health concern, as animals serve as reservoirs for pathogens, threatening food safety, biodiversity, and human health. In response, novel antiviral strategies are urgently needed. This study investigates the antiviral activity and structural properties of two antimicrobial peptides, pantinin-1 and pantinin-2, both derived from the venom of the scorpion Pandinus imperator, against caprine herpesvirus 1 (CpHV-1) and bovine herpesvirus 1 (BoHV-1). The results obtained from the plaque reduction assay and the quantitative real-time polymerase chain reaction (PCR) indicate that synthetic pantinin-mimetic peptides exhibited potent antiviral effects at concentrations ranging from 6-25 µM, impairing viral infectivity through direct virucidal action and inhibition of the viral entry and fusion with host cell. To characterize their structural behavior, nuclear magnetic resonance spectroscopy is performed in aqueous and membrane-mimetic environments (trifluoroethanol (TFE)/H2O). In aqueous solution, both peptides predominantly adopted random coil conformations, with pantinin-2 showing greater secondary structure propensity. In TFE/H2O, both peptides transitioned to α-helical structures, which are often associated with membrane interaction and antiviral activity. These findings demonstrate that pantinin-1 and pantinin-2 possess promising antiviral properties, supporting their potential development as therapeutic agents against herpesviruses and other animal viral infections.

抗兽医疱疹病毒的潘汀衍生肽:活性和结构表征。
动物病毒感染是一个日益严重的公共卫生问题,因为动物是病原体的宿主,威胁着食品安全、生物多样性和人类健康。因此,迫切需要新的抗病毒策略。本研究研究了两种抗菌肽pantinin-1和pantinin-2对山羊疱疹病毒1 (CpHV-1)和牛疱疹病毒1 (BoHV-1)的抗病毒活性和结构特性。斑块减少实验和实时定量聚合酶链反应(PCR)结果表明,合成的pantinin模拟肽在6-25µM的浓度范围内表现出强大的抗病毒作用,通过直接杀死病毒和抑制病毒进入和与宿主细胞融合来削弱病毒的感染性。为了表征它们的结构行为,在水和膜模拟环境(三氟乙醇(TFE)/H2O)中进行核磁共振波谱。在水溶液中,这两种肽主要采用随机卷曲构象,其中pantinin-2具有更大的二级结构倾向。在TFE/H2O中,两种肽都转变为α-螺旋结构,这通常与膜相互作用和抗病毒活性有关。这些发现表明,pantinin-1和pantinin-2具有良好的抗病毒特性,支持它们作为疱疹病毒和其他动物病毒感染的治疗药物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ChemMedChem
ChemMedChem 医学-药学
CiteScore
6.70
自引率
2.90%
发文量
280
审稿时长
1 months
期刊介绍: Quality research. Outstanding publications. With an impact factor of 3.124 (2019), ChemMedChem is a top journal for research at the interface of chemistry, biology and medicine. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. ChemMedChem publishes primary as well as critical secondary and tertiary information from authors across and for the world. Its mission is to integrate the wide and flourishing field of medicinal and pharmaceutical sciences, ranging from drug design and discovery to drug development and delivery, from molecular modeling to combinatorial chemistry, from target validation to lead generation and ADMET studies. ChemMedChem typically covers topics on small molecules, therapeutic macromolecules, peptides, peptidomimetics, and aptamers, protein-drug conjugates, nucleic acid therapies, and beginning 2017, nanomedicine, particularly 1) targeted nanodelivery, 2) theranostic nanoparticles, and 3) nanodrugs. Contents ChemMedChem publishes an attractive mixture of: Full Papers and Communications Reviews and Minireviews Patent Reviews Highlights and Concepts Book and Multimedia Reviews.
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