Synthesis and characterization of calcium-containing sodium cyclotriphosphate and its protective effect on enamel erosion in vitro.

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-09-12 DOI:10.1016/j.dental.2025.09.001

Marcela Macedo Nunes, Luigi Pedrini Guisso, Thayse Yumi Hosida, Emerson Rodrigues de Camargo, João Carlos Silos Moraes, Juliano Pelim Pessan, Denise Pedrini, Alberto Carlos Botazzo Delbem

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引用次数: 0

Abstract

Objective: The objective of the present study was to synthesize and characterize sodium cyclotriphosphate (NaTMP) containing calcium and verify its effect using an initial enamel erosion model.

Methods: Cyclotriphosphate containing calcium (CaNaTMP) was synthesized using column chromatography, and addition of a solution with calcio hydroxide supernatant and analyzed by scanning electron microscopy and energy-dispersive X-ray spectroscopy. To determine the effect on enamel initial erosion, sound bovine enamel blocks (n = 96) were selected by initial surface hardness and divided into to 8 experimental groups (12 blocks/group): control (deionized water), 0.24 % NaF (1100 F), 0.25 %, 0.5 % and 1 % NaTMP and CaNaTMP at the same concentrations. The enamel blocks were immersed in 4 mL of experimental solutions for 2 min followed by 4 erosive challenges (citric acid, 0.75 %, pH 3.5, for 1 min, under stirring). The surface hardness was determined after each acid challenge. Data were subjected to two-way repeated measures analysis of variance, followed by Tukey's test (p < 0.05).

Results: The synthesis process led to the replacement of atoms of Na by atoms of Ca with particle sizes like those of NaTMP. Solutions containing CaNaTMP promoted superior reducing in hardness loss when compared to their counterparts without calcium (p < 0.001) and 1100 F (p < 0.001), during erosive challenges. A dose-response was observed for the CaNaTMP groups, with higher values for 1 % CaNaTMP and lower values for 0.25 % CaNaTMP (p < 0.001).

Significance: Solutions containing CaNaTMP led to superior protective effects compared to the 1100 F group on initial enamel erosion.

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含钙环三磷酸钠的合成、表征及其对牙釉质腐蚀的保护作用。

目的：合成含钙的环三磷酸钠（NaTMP）并对其进行表征，并通过初始牙釉质侵蚀模型验证其效果。方法：采用柱层析法合成含钙环三磷酸（CaNaTMP），并加入氢氧化钙上清溶液，通过扫描电镜和x射线能谱分析。为了确定对牙釉质初始侵蚀的影响，根据初始表面硬度选择牛牙釉质块（n = 96），分为8个实验组（12块/组）：对照组（去离子水）、0.24 % NaF（1100 F）、0.25 %、0.5 %和1 %相同浓度的NaTMP和CaNaTMP。牙釉质块在4 mL实验溶液中浸泡2 min，然后进行4次侵蚀（柠檬酸，0.75 %,pH 3.5，搅拌1 min）。每次酸浸后测定表面硬度。数据进行双向重复测量方差分析，然后进行Tukey检验（p ）结果：合成过程导致Na原子被Ca原子取代，其粒径与NaTMP相似。与不含钙的溶液相比，含有CaNaTMP的溶液对硬度损失的降低效果更好(p 意义：与1100 F组相比，含有CaNaTMP的溶液对初始牙釉质侵蚀的保护作用更好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.