Temperature Detection in Individual Brown Adipocytes with Nitrogen-Vacancy Ensemble in Diamond

IF 4.3 Q1 OPTICS
Penglong Li, Xin Li, Xiaojiang Wang, Zhuowen Chen, Huan Fei Wen, Zongmin Ma, Jun Tang, Jun Liu
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引用次数: 0

Abstract

Brown adipocytes participate in regulating body temperature through heat production, and temperature measurement is essential for understanding their thermogenesis mechanism. Although many advanced techniques have been proposed, achieving non-invasive temperature detection at the single-cell level still presents significant challenges. Here, a method is developed to measure the extracellular temperature of cultured brown adipocytes using nitrogen-vacancy ensemble in diamond. The measurement principle is based on the resonance frequency of nitrogen-vacancy centers changing with temperature when heat flows into the diamond integrated with nitrogen-vacancy ensemble. This method enables precise imaging the temperature distribution differences on individual cell surfaces with a temperature sensitivity of 35 mK Hz−1/2. With this method, a temperature increase of over 0.5 °C is observed in brown adipocytes stimulated by mitochondrial uncoupling agents. This result demonstrates that NV ensemble is a promising tool for studying thermogenesis in brown adipocytes and provides new research approaches for non-invasive cell temperature measurements.

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金刚石中含氮空位系综的单个棕色脂肪细胞的温度检测
棕色脂肪细胞通过产热参与调节体温,温度测量是了解其产热机制的必要条件。尽管已经提出了许多先进的技术,但在单细胞水平上实现无创温度检测仍然存在重大挑战。本文建立了一种利用金刚石中氮空位系综测量培养棕色脂肪细胞胞外温度的方法。测量原理是基于热流进氮-空位系综金刚石时氮-空位中心共振频率随温度变化的原理。该方法能够精确成像单个细胞表面的温度分布差异,温度灵敏度为35 mK Hz−1/2。用这种方法,观察到在线粒体解偶联剂刺激下棕色脂肪细胞的温度升高超过0.5°C。这一结果表明,NV系综是研究棕色脂肪细胞产热的一种很有前途的工具,并为非侵入性细胞温度测量提供了新的研究方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
7.90
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0.00%
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