Penglong Li, Xin Li, Xiaojiang Wang, Zhuowen Chen, Huan Fei Wen, Zongmin Ma, Jun Tang, Jun Liu
{"title":"Temperature Detection in Individual Brown Adipocytes with Nitrogen-Vacancy Ensemble in Diamond","authors":"Penglong Li, Xin Li, Xiaojiang Wang, Zhuowen Chen, Huan Fei Wen, Zongmin Ma, Jun Tang, Jun Liu","doi":"10.1002/qute.202400664","DOIUrl":null,"url":null,"abstract":"<p>Brown adipocytes participate in regulating body temperature through heat production, and temperature measurement is essential for understanding their thermogenesis mechanism. Although many advanced techniques have been proposed, achieving non-invasive temperature detection at the single-cell level still presents significant challenges. Here, a method is developed to measure the extracellular temperature of cultured brown adipocytes using nitrogen-vacancy ensemble in diamond. The measurement principle is based on the resonance frequency of nitrogen-vacancy centers changing with temperature when heat flows into the diamond integrated with nitrogen-vacancy ensemble. This method enables precise imaging the temperature distribution differences on individual cell surfaces with a temperature sensitivity of 35 mK Hz<sup>−1/2</sup>. With this method, a temperature increase of over 0.5 °C is observed in brown adipocytes stimulated by mitochondrial uncoupling agents. This result demonstrates that NV ensemble is a promising tool for studying thermogenesis in brown adipocytes and provides new research approaches for non-invasive cell temperature measurements.</p>","PeriodicalId":72073,"journal":{"name":"Advanced quantum technologies","volume":"8 9","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced quantum technologies","FirstCategoryId":"1085","ListUrlMain":"https://advanced.onlinelibrary.wiley.com/doi/10.1002/qute.202400664","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
Brown adipocytes participate in regulating body temperature through heat production, and temperature measurement is essential for understanding their thermogenesis mechanism. Although many advanced techniques have been proposed, achieving non-invasive temperature detection at the single-cell level still presents significant challenges. Here, a method is developed to measure the extracellular temperature of cultured brown adipocytes using nitrogen-vacancy ensemble in diamond. The measurement principle is based on the resonance frequency of nitrogen-vacancy centers changing with temperature when heat flows into the diamond integrated with nitrogen-vacancy ensemble. This method enables precise imaging the temperature distribution differences on individual cell surfaces with a temperature sensitivity of 35 mK Hz−1/2. With this method, a temperature increase of over 0.5 °C is observed in brown adipocytes stimulated by mitochondrial uncoupling agents. This result demonstrates that NV ensemble is a promising tool for studying thermogenesis in brown adipocytes and provides new research approaches for non-invasive cell temperature measurements.