Circ-ATF7IP promotes IL13RA1 dependent M2 polarization of macrophage via sponging MiR-488-3p in papillary thyroid carcinoma

Abstract

The preoperative assessment of lymph node metastasis (LNM) is of critical importance for the selection of appropriate surgical and therapeutic strategies for papillary thyroid carcinoma (PTC). However, the mechanisms underlying LNM in PTC remain unclear, and effective diagnostic tools are currently lacking. This study aims to identify circRNAs as diagnostic molecular markers for LNM and to investigate their molecular mechanisms in the occurrence of LNM in PTC. In this study, bioinformatics analysis and qPCR verification were used and identified that circ-ATF7IP was the key molecule in PTC progression and overexpressed in PTC patients with LNM. Mechanistically, circ-ATF7IP acts as a molecular sponge for miR-488-3p in PTC cells. PTC cell-derived miR-488-3p acts on macrophage (Mɸ) through exosome transfer. In Mɸ, miR-488-3p negatively regulates IL13RA1 at post transcription stage, facilitating M2 phenotype polarization. This reshapes the tumor microenvironment, enhancing tumor invasiveness. To be brief, circ-ATF7IP is a newly identified biomarker for PTC related LNM. Targeting circ-ATF7IP/miR-488-3p/IL13RA1 axis is a promising therapeutic strategy for PTC.