Drug repurposing with non-antibiotic strategies against S. aureus and molecular profiling of resistance genes in Nile tilapia

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-11 DOI:10.1016/j.micpath.2025.108033

Laiba Shafique , Peng Zhu , Youhou Xu , Wardah Hassan , Fariha Latif , Muhammad Abdul Manan , Shakeela Parveen , Muhammad Farhan Khan

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引用次数: 0

Abstract

Staphylococcus aureus is a major food-borne opportunistic pathogen that poses a significant public health threat, leading to severe tissue infections, bacteremia, and often life-threatening illness. While S. aureus has been extensively studied in livestock and poultry products in China, there is a notable lack of data regarding its presence and characteristics in aquatic products. This study investigates the prevalence, antibiotic resistance (ABR), its molecular profiling, and treatment regimens of S. aureus isolates from Oreochromis niloticus (Nile tilapia). A total of 300 tilapia samples from various fish markets showed an overall S. aureus prevalence of 31.67 %. ABR profiles revealed significant resistance to commonly used antibiotics, such as amoxicillin/clavulanic acid (65 %) and tetracycline (55 %), highlighting the widespread emergence of resistance. Phylogenetic analysis revealed strong clade support for resistance gene clusters, such as MecA/C, ErmA, and TetK. Motif analysis showed distinct motifs, indicating their role in ABR mechanisms. Physiochemical properties showed that β-lactams and macrolides have a hydrophilic nature, and the tetracycline class exhibited a hydrophobic nature. BlaZ and MecA exhibit the highest occurrence of GATA, potentially emphasizing the regulation by transcription factors in the resistance to β-lactams group, while TEM exhibited a high frequency of GATA, YY1, and OCT1, implying these factors may regulate β-lactamase production. Through synergy evolution, one synergistic and three additive interactions were identified, indicating its potential for novel combination therapies against multidrug-resistant (MDR) pathogens. Our study findings underscore the severity of ABR in aquaculture, highlighting the importance of effective antibiotic strategies. The comparative analysis of treatment efficacy also highlights the need for alternative strategies to control bacterial infections. Overall, our work offers valuable insights into resistance mechanisms and future management approaches for combating MDR pathogens in aquaculture settings.

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尼罗罗非鱼抗金黄色葡萄球菌非抗生素药物再利用及耐药基因分子谱分析

金黄色葡萄球菌是一种主要的食源性机会性病原体，对公共卫生构成重大威胁，导致严重的组织感染、菌血症和经常危及生命的疾病。虽然金黄色葡萄球菌在中国的畜禽产品中得到了广泛的研究，但关于其在水产品中的存在和特征的数据明显缺乏。研究了尼罗罗非鱼（Oreochromis niloticus）金黄色葡萄球菌的流行、抗生素耐药性（ABR）、分子谱和治疗方案。来自各鱼市的300份罗非鱼样本显示，金黄色葡萄球菌的总体流行率为31.67%。ABR谱显示对常用抗生素，如阿莫西林/克拉维酸（65%）和四环素（55%）具有显著耐药性，突出表明耐药性的广泛出现。系统发育分析显示，耐药基因簇如MecA/C、ErmA和TetK具有很强的进化支系支持。基序分析显示不同的基序，表明它们在ABR机制中所起的作用。理化性质表明，β-内酰胺类和大环内酯类具有亲水性，而四环素类具有疏水性。BlaZ和MecA中GATA的发生率最高，可能强调了转录因子对β-内酰胺耐药组的调控作用，而TEM中GATA、YY1和OCT1的表达频率较高，暗示这些因子可能调控β-内酰胺酶的产生。通过协同进化，确定了一种协同作用和三种加性相互作用，表明其具有针对多药耐药（MDR）病原体的新型联合治疗的潜力。我们的研究结果强调了水产养殖中ABR的严重性，强调了有效抗生素策略的重要性。治疗效果的比较分析也强调了控制细菌感染的替代策略的必要性。总的来说，我们的工作为在水产养殖环境中对抗耐多药病原体的耐药机制和未来管理方法提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)