Quantum dot-based fluorescence-linked immunosorbent assay for high-throughput detection of apramycin residues in animal-derived foods

IF 6.3 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control Pub Date : 2025-09-05 DOI:10.1016/j.foodcont.2025.111709

Chao Liang , Haoyu Huang , Wenjing Chen , Hongliang Liu , Jingming Zhou , Yumei Chen , Xifang Zhu , Enping Liu , Aiping Wang

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引用次数: 0

Abstract

Apramycin (APR), a widely used broad-spectrum aminoglycoside antibiotic, poses potential risks to human health due to residue accumulation in animal-derived foods. Therefore, high-throughput rapid detection methods with high sensitivity and low cost advantages are urgently needed to implemented to safeguard public health. In this study, high-affinity and target-selective monoclonal antibody (mAb) against APR was developed, and a high-sensitivity quantum dot-based direct competitive fluorescence-linked immunosorbent assay (dc-FLISA) was established for rapid APR detection. The dc-FLISA demonstrated a 50 % inhibition concentration (IC₅₀) of 3.91 ng/mL, a limit of detection (LOD) of 0.38 ng/mL, and a linear range of 0.68–22.39 ng/mL. Recoveries from spiked food samples ranged from 94.3 % to 108.3 %, with coefficients of variation (CV) less than 10 %. This established method combines high sensitivity, cost-effectiveness, and rapid detection, offering a reliable technical solution for large-scale detection and regulatory compliance monitoring of APR livestock-derived food products, ultimately safeguarding consumer health.

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基于量子点荧光联免疫吸附法的动物源性食品中阿帕霉素残留高通量检测

阿帕霉素（APR）是一种广泛使用的广谱氨基糖苷类抗生素，由于其在动物源性食品中的残留积累，对人类健康构成潜在风险。因此，迫切需要实现高灵敏度、低成本优势的高通量快速检测方法，以保障公众健康。本研究开发了针对APR的高亲和力和靶向选择性单克隆抗体（mAb），并建立了基于量子点的高灵敏度直接竞争荧光联免疫吸附法（dc-FLISA），用于快速检测APR。dc-FLISA的50%抑制浓度（IC50）为3.91 ng/mL，检测限（LOD）为0.38 ng/mL，线性范围为0.68 ~ 22.39 ng/mL。加标食品样品的回收率为94.3% ~ 108.3%，变异系数小于10%。该方法具有灵敏度高、成本效益好、检测速度快等特点，为APR畜产食品的大规模检测和合规监测提供了可靠的技术解决方案，最终保障了消费者的健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.