Eva Fellinger , Tom Brandt , Andrea Schittenhelm , Eric Quarg , Matthias Pröll , Gregor Domes , Annette Schmidt
{"title":"Virtual stress testing: Analyzing endocrine, metabolic, cardiovascular, and psychological responses to the TSST-VR","authors":"Eva Fellinger , Tom Brandt , Andrea Schittenhelm , Eric Quarg , Matthias Pröll , Gregor Domes , Annette Schmidt","doi":"10.1016/j.ynstr.2025.100760","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>The Trier Social Stress Test (TSST) is a widely used tool for inducing and measuring stress responses in a controlled environment. In this study, we aimed to explore the effectiveness of a virtual TSST (TSST-VR) in eliciting stress responses across multiple physiological and psychological markers.</div></div><div><h3>Methods</h3><div>A sample of 24 participants underwent the TSST-VR, during which salivary cortisol, alpha-amylase (AA), blood glucose levels, heart rate (HR), root mean square of successive differences (RMSSD) as a measure of heart rate variability (HRV), and subjective stress ratings (NRS) were collected at multiple time points.</div></div><div><h3>Results</h3><div>In a baseline-to-peak analysis, significant increases were observed in HR (M<sub>Diff</sub> = 13.04, 95 %-CI [8.19–17.90], p < .001), RMSSD (M<sub>Diff</sub> = 17.75, 95 %-CI [3.28–32.22], p < .001), AA (p < .001, r = 1.07), and NRS (p < .001, r = 1.31) measures following the TSST-VR. While no significant changes in cortisol levels were found in the baseline-to-peak analysis across all participants, a secondary cluster analysis identified distinct cortisol responders (baseline-to-peak rise >1.5 mmol/l). Within this group, high cortisol responders (HCR) showed significantly higher cortisol (Wald χ<sup>2</sup>(7) = 118.03, p < .001), HR (Wald χ<sup>2</sup>(8) = 17.91, p = .022), and AA levels (Wald χ<sup>2</sup>(7) = 17.13, p = .017) compared to low cortisol responders (LCR). Area-under-the-curve analysis further confirmed a more robust cortisol stress response in HCR.</div></div><div><h3>Conclusion</h3><div>These findings suggest that the TSST-VR can effectively induce measurable stress responses and may provide insights into individual differences in physiological and metabolic stress reactions. The study highlights the potential of virtual stress paradigms in stress research and underscores the advantages of a virtual setting in terms of standardization and economic considerations.</div></div>","PeriodicalId":19125,"journal":{"name":"Neurobiology of Stress","volume":"39 ","pages":"Article 100760"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurobiology of Stress","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352289525000542","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background
The Trier Social Stress Test (TSST) is a widely used tool for inducing and measuring stress responses in a controlled environment. In this study, we aimed to explore the effectiveness of a virtual TSST (TSST-VR) in eliciting stress responses across multiple physiological and psychological markers.
Methods
A sample of 24 participants underwent the TSST-VR, during which salivary cortisol, alpha-amylase (AA), blood glucose levels, heart rate (HR), root mean square of successive differences (RMSSD) as a measure of heart rate variability (HRV), and subjective stress ratings (NRS) were collected at multiple time points.
Results
In a baseline-to-peak analysis, significant increases were observed in HR (MDiff = 13.04, 95 %-CI [8.19–17.90], p < .001), RMSSD (MDiff = 17.75, 95 %-CI [3.28–32.22], p < .001), AA (p < .001, r = 1.07), and NRS (p < .001, r = 1.31) measures following the TSST-VR. While no significant changes in cortisol levels were found in the baseline-to-peak analysis across all participants, a secondary cluster analysis identified distinct cortisol responders (baseline-to-peak rise >1.5 mmol/l). Within this group, high cortisol responders (HCR) showed significantly higher cortisol (Wald χ2(7) = 118.03, p < .001), HR (Wald χ2(8) = 17.91, p = .022), and AA levels (Wald χ2(7) = 17.13, p = .017) compared to low cortisol responders (LCR). Area-under-the-curve analysis further confirmed a more robust cortisol stress response in HCR.
Conclusion
These findings suggest that the TSST-VR can effectively induce measurable stress responses and may provide insights into individual differences in physiological and metabolic stress reactions. The study highlights the potential of virtual stress paradigms in stress research and underscores the advantages of a virtual setting in terms of standardization and economic considerations.
期刊介绍:
Neurobiology of Stress is a multidisciplinary journal for the publication of original research and review articles on basic, translational and clinical research into stress and related disorders. It will focus on the impact of stress on the brain from cellular to behavioral functions and stress-related neuropsychiatric disorders (such as depression, trauma and anxiety). The translation of basic research findings into real-world applications will be a key aim of the journal.
Basic, translational and clinical research on the following topics as they relate to stress will be covered:
Molecular substrates and cell signaling,
Genetics and epigenetics,
Stress circuitry,
Structural and physiological plasticity,
Developmental Aspects,
Laboratory models of stress,
Neuroinflammation and pathology,
Memory and Cognition,
Motivational Processes,
Fear and Anxiety,
Stress-related neuropsychiatric disorders (including depression, PTSD, substance abuse),
Neuropsychopharmacology.