Apoferritin-Functionalized with Wheat Germ Agglutinin and Loaded with Antibiotic for Targeting Bacteria.

Q2 Biochemistry, Genetics and Molecular Biology

Iranian Biomedical Journal Pub Date : 2025-06-25 DOI:10.61186/ibj.5039

Qristine G Gyurjyan, Gayane G Poghosyan, Vardan K Gasparyan

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Abstract

Background: The selective delivery of drugs to their targets prevents their possible side effects; hence, the development of selective transport systems is considered extremely promising. In the present study, we aimed to develop a drug delivery system for targeting bacteria.

Methods: Functionalization of apoferritin with WGA, as a bacteria-recognizing lectin, was conducted. Afterwards, the complex was loaded with ampicillin. The affinity of the conjugate to Gram-positive bacteria, B. subtilis, was evaluated by anisotropic AgNPs conjugated with this complex, and its interaction with the bacteria was also assessed.

Results: The drug-delivery capabilities of the composite were evaluated. Results from the disk diffusion assay revealed that its bactericidal activity is 10-fold greater compared to free antibiotics. The effectiveness of the bactericidal effects of ampicillin-loaded ferritin was also confirmed in whole blood.

Conclusion: Lectin-conjugated and ampicillin-loaded apoferritin can be considered as effective drug delivery systems for targeting bacteria.

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用小麦胚芽凝集素功能化载铁蛋白并装载抗生素靶向细菌。

背景：选择性地将药物递送到其目标可以防止其可能的副作用；因此，选择性运输系统的发展被认为是非常有前途的。在本研究中，我们旨在开发一种靶向细菌的药物传递系统。方法：采用WGA作为细菌识别凝集素，对载铁蛋白进行功能化。之后，该复合体被加载氨苄西林。通过各向异性AgNPs偶联物对革兰氏阳性细菌枯草芽孢杆菌的亲和力进行了评价，并对其与细菌的相互作用进行了评价。结果：评价了该复合物的给药能力。圆盘扩散试验结果表明，其杀菌活性比游离抗生素高10倍。在全血中也证实了氨苄西林载铁蛋白的杀菌效果。结论：凝集素偶联和氨苄西林载铁蛋白可作为有效的靶向细菌给药系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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