¹H, ¹³C and ¹⁵N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1-2).

IF 0.6 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-09-13 DOI:10.1007/s12104-025-10248-z

Florian Malard, Léa Bouton, Sébastien Campagne

引用次数: 0

Abstract

The N-terminal tandem RNA Recognition Motifs (RRM1-2) of the splicing factor RNA Binding Motif 39 (RBM39) from Homo sapiens was expressed in E. coli with ¹³C and ¹⁵N labeling. A nearly complete assignment of backbone ¹H, ¹⁵N, and ¹³C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1-2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.

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RNA结合基序39 （RBM39）串联RNA识别基序（RRM1-2）的1H， 13C和15N骨干共振分配。

用13C和15N标记在大肠杆菌中表达了来自人类的剪接因子RNA结合Motif 39 （RBM39）的n端串联RNA识别Motif （RRM1-2）。获得了几乎完整的主链1H、15N和13C共振，以及基于分配的化学位移的二级结构预测。预测的RBM39串联RRM1-2的二级结构与已发表的单个RBM39 RRM1和RRM2的溶液结构非常吻合，并且与RBM39的AlphaFold2模型吻合良好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.

1H, 13C and 15N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1-2).

Abstract

¹H, ¹³C and ¹⁵N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1-2).