Comparing the impacts of aerosolization and sampling techniques on the structural integrity and antigenicity retention of influenza A virus-like particles

IF 2.9 3区 环境科学与生态学 Q2 ENGINEERING, CHEMICAL
Yuezhi (August) Li , Ananya Benegal , Joseph V. Puthussery , Shu-Wen You , Michael D. Vahey , Rajan K. Chakrabarty
{"title":"Comparing the impacts of aerosolization and sampling techniques on the structural integrity and antigenicity retention of influenza A virus-like particles","authors":"Yuezhi (August) Li ,&nbsp;Ananya Benegal ,&nbsp;Joseph V. Puthussery ,&nbsp;Shu-Wen You ,&nbsp;Michael D. Vahey ,&nbsp;Rajan K. Chakrabarty","doi":"10.1016/j.jaerosci.2025.106673","DOIUrl":null,"url":null,"abstract":"<div><div>Laboratory experiments studying respiratory virus aerosols rely on the reproducibility of aerosolization and sampling techniques. Conventional techniques could compromise viral structure and antigenicity, particularly for pleomorphic viruses like influenza A (IAV), yet very little information is available on this issue. Here, we evaluate three aerosolization methods: Collison, Blaustein Atomization Modules (BLAM), and jet nebulizers, and three bioaerosol samplers: liquid spot sampler (LSS), wet cyclone, and SKC BioSampler, to determine their efficiency in retaining the structural stability and antigenicity of filamentous IAV virus-like particles (VLPs). VLPs provide a safe and practical alternative for studying highly pathogenic airborne viruses. The BLAM and jet nebulizers maintain 12–21 % of filamentous structures, whereas the Collison nebulizer, which generates higher shear stress, reduces filament recovery to ∼10 %. The liquid spot sampler (LSS), owing to gentle condensation-based sampling technique, retains approximately 30 % of filamentous VLPs. The SKC BioSampler and wet cyclone sampler cause greater structural disruption due to higher shear stress and impaction forces and retain ∼10 % and ∼7 % of filamentous VLPs, respectively. Higher relative humidity (85 %) improves filament retention by ∼20 % compared to dry conditions (25 % RH). The antigenicity of Neuraminidase (NA), the IAV surface protein responsible for viral release, followed a bimodal distribution, with up to 20 % of small VLPs showing undetectable NA signal post-aerosolization, indicating greater susceptibility to structural degradation. These results point to the necessity of improving upon contemporary aerosolization and sampling strategies to characterize airborne filamentous viruses in controlled laboratory environments more accurately.</div></div>","PeriodicalId":14880,"journal":{"name":"Journal of Aerosol Science","volume":"191 ","pages":"Article 106673"},"PeriodicalIF":2.9000,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Aerosol Science","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021850225001508","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Laboratory experiments studying respiratory virus aerosols rely on the reproducibility of aerosolization and sampling techniques. Conventional techniques could compromise viral structure and antigenicity, particularly for pleomorphic viruses like influenza A (IAV), yet very little information is available on this issue. Here, we evaluate three aerosolization methods: Collison, Blaustein Atomization Modules (BLAM), and jet nebulizers, and three bioaerosol samplers: liquid spot sampler (LSS), wet cyclone, and SKC BioSampler, to determine their efficiency in retaining the structural stability and antigenicity of filamentous IAV virus-like particles (VLPs). VLPs provide a safe and practical alternative for studying highly pathogenic airborne viruses. The BLAM and jet nebulizers maintain 12–21 % of filamentous structures, whereas the Collison nebulizer, which generates higher shear stress, reduces filament recovery to ∼10 %. The liquid spot sampler (LSS), owing to gentle condensation-based sampling technique, retains approximately 30 % of filamentous VLPs. The SKC BioSampler and wet cyclone sampler cause greater structural disruption due to higher shear stress and impaction forces and retain ∼10 % and ∼7 % of filamentous VLPs, respectively. Higher relative humidity (85 %) improves filament retention by ∼20 % compared to dry conditions (25 % RH). The antigenicity of Neuraminidase (NA), the IAV surface protein responsible for viral release, followed a bimodal distribution, with up to 20 % of small VLPs showing undetectable NA signal post-aerosolization, indicating greater susceptibility to structural degradation. These results point to the necessity of improving upon contemporary aerosolization and sampling strategies to characterize airborne filamentous viruses in controlled laboratory environments more accurately.
比较雾化和采样技术对甲型流感病毒样颗粒结构完整性和抗原性保留的影响
研究呼吸道病毒气溶胶的实验室实验依赖于雾化和采样技术的可重复性。传统技术可能会损害病毒的结构和抗原性,特别是对于像甲型流感(IAV)这样的多形性病毒,但关于这一问题的信息很少。在这里,我们评估了三种雾化方法:Collison、Blaustein雾化模块(BLAM)和喷射雾化器,以及三种生物气溶胶采样器:液体斑点采样器(LSS)、湿气旋采样器和SKC生物采样器,以确定它们在保持丝状IAV病毒样颗粒(VLPs)结构稳定性和抗原性方面的效率。VLPs为研究高致病性空气传播病毒提供了一种安全实用的方法。BLAM喷雾器和喷射喷雾器保持了12 - 21%的丝状结构,而Collison喷雾器产生更高的剪切应力,将丝状结构的回复率降低到10%。液体斑点采样器(LSS),由于温和的冷凝为基础的采样技术,保留了约30%的丝状VLPs。SKC生物采样器和湿旋流采样器由于更高的剪切应力和冲击力导致更大的结构破坏,分别保留了10%和7%的丝状VLPs。与干燥条件(25% RH)相比,较高的相对湿度(85%)可使长丝保持率提高约20%。神经氨酸酶(NA)是负责病毒释放的IAV表面蛋白,其抗原性遵循双峰分布,高达20%的小vlp在雾化后显示无法检测到NA信号,表明更容易受到结构降解。这些结果表明,有必要改进当代雾化和采样策略,以便在受控的实验室环境中更准确地表征空气中的丝状病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Aerosol Science
Journal of Aerosol Science 环境科学-工程:化工
CiteScore
8.80
自引率
8.90%
发文量
127
审稿时长
35 days
期刊介绍: Founded in 1970, the Journal of Aerosol Science considers itself the prime vehicle for the publication of original work as well as reviews related to fundamental and applied aerosol research, as well as aerosol instrumentation. Its content is directed at scientists working in engineering disciplines, as well as physics, chemistry, and environmental sciences. The editors welcome submissions of papers describing recent experimental, numerical, and theoretical research related to the following topics: 1. Fundamental Aerosol Science. 2. Applied Aerosol Science. 3. Instrumentation & Measurement Methods.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信