Insight into using a novel ultrasound followed by peracetic acid / ultraviolet disinfection system to inactivate microorganisms in sewage based on synergy and metagenomics

IF 6.7 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-09-12 DOI:10.1016/j.jwpe.2025.108662

Zhi Zhang , Yun Bai , Ruiting Chang , Xueli Hu , Juanjuan Yao

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Abstract

During the epidemic (SARS-CoV-2 virus), although chlorination played a vital role in disinfecting sewage of isolated areas, excessive chlorine disinfectants also led to serious secondary pollution. In order to avoid secondary pollution caused by traditional disinfection, we constructed a sequential ultrasonic combined with peracetic acid/ultraviolet (US→PAA/UV) system to disinfect actual sewage. The result indicated that the US→PAA/UV had an excellent synergistic disinfection effect on coliform, reaching 2.78-log, which was higher than the sum of the reductions achieved by ultrasound (0.04-log), UV (1.93-log) and PAA (0.36-log). Furthermore, metagenomic sequencing showed that US→PAA/UV treatment could significantly reduce the relative abundance of opportunistic pathogens, specifically Acinetobacter (7.22 % to 5.61 %), Moraxella (7.11 % to 1.38 %) and Cloacibacterium (3.5 % to 1.68 %). This reduction was achieved by disrupting the normal expression of several functional genes (TraI, LuxR, mqsR, lasR and galE) involved in quorum sensing and extracellular polymeric substance (EPS) secretion. In addition, the antibiotics resistance gene (ARGs) analysis based on CARD database found that the number of ARGs subtypes changed little, with 921 and 927, respectively, before and after US→PAA/UV. Overall, The results demonstrated that US→PAA/UV could not only effectively inactivate multiple pathogens but also pose no risk of increasing bacterial resistance, providing new insights for the emergency disinfection of raw sewage during public health emergencies.

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基于协同和宏基因组学的新型超声后过氧乙酸/紫外线消毒系统灭活污水中的微生物的研究

在疫情期间（SARS-CoV-2病毒），虽然氯化对隔离区污水消毒起到了至关重要的作用，但含氯消毒剂过量也导致了严重的二次污染。为避免传统消毒造成的二次污染，构建了超声-过氧乙酸/紫外线（US→PAA/UV）序贯系统对实际污水进行消毒。结果表明，US→PAA/UV对大肠菌群具有良好的协同消毒效果，达到2.78 log，高于超声（0.04 log）、UV （1.93 log）和PAA （0.36 log）的减毒效果之和。此外，宏基因组测序结果显示，US→PAA/UV处理可显著降低条件致病菌的相对丰度，特别是不动杆菌（7.22% ~ 5.61%）、莫拉菌（7.11% ~ 1.38%）和Cloacibacterium（3.5% ~ 1.68%）。这种减少是通过破坏几个参与群体感应和细胞外聚合物（EPS）分泌的功能基因（TraI, LuxR, mqsR， lasR和galE）的正常表达来实现的。此外，基于CARD数据库的抗生素耐药基因（ARGs）分析发现，US→PAA/UV前后ARGs亚型数量变化不大，分别为921个和927个。综上所示，US→PAA/UV不仅能有效灭活多种病原菌，而且不会增加细菌耐药性，为突发公共卫生事件中原污水的应急消毒提供了新的思路。

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来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies