Phytochemical investigation of Commiphora stocksiana (Engl.) Engl. and its comparative metabolomic study with Commiphora wightii (Arn.) Bhandari. Using HPLC-MS-Qtof

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-09-12 DOI:10.1016/j.bse.2025.105122

Shivani Bhati, Aruna Joshi

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Abstract

The oleo-gum resins and essential oils from genus Commiphora Jacq. have been widely used commercially for different medicinal purposes all over the world. This study presents a tentative phytochemical profile of one of its lesser-known endangered species Commiphora stocksiana (Engl.) Engl. and a comparative assessment with Commiphora wightii (Arn.) Bhandari stem fractions. The fractionation was performed across three different solvent polarities; analysis of the fractions were performed using HPLC-MS-QToF in both positive and negative electrospray ionization (ESI) modes. The statistical analysis performed for the comparative assessment of the two species revealed that methanolic fractions displayed the most pronounced species-level separation, followed by moderate differentiation in ethyl acetate fractions, while petroleum ether fractions exhibited considerable overlap, indicating compositional similarity. The spectral library-based comparison of the features observed in each fraction led to tentative identification of 61 metabolites of varying compound classes in C. stocksiana. Further MS/MS fragmentation analysis of some features observed in petroleum ether fraction of C. stocksiana revealed the presence of Guggulsterone E, confirmed by alignment with a positive control from C. wightii using exact ion chromatogram tool in MS-DIAL. These findings were verified using HPLC analysis by using reference standard mix of Guggulsterone E and Z isomer. Additional feature showed retro-Diels-Alder (RDA) type cleavage of precursor ions, suggesting the presence of ursane- and oleanane-type pentacyclic triterpenoids, highly similar to the boswellic acid derivatives.

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黄花香薷植物化学研究（英文版）心血管病。及其与白粉虱的比较代谢组学研究班达里。使用HPLC-MS-Qtof

芫花属的油胶树脂和精油。在世界各地被广泛用于不同的商业药用目的。本研究提出了一种不太为人所知的濒危物种——stocksiana （Engl.）的植物化学特征。心血管病。并与麻蝇（comiphora wightii, Arn.）进行比较评价。班达里茎分数。在三种不同的溶剂极性下进行分馏；采用HPLC-MS-QToF在正、负电喷雾电离（ESI）模式下对各组分进行分析。对两种植物进行比较分析的统计分析表明，甲醇馏分表现出最明显的种水平分离，其次是乙酸乙酯馏分的中等分化，而石油醚馏分表现出相当大的重叠，表明成分相似。基于光谱文库对各组分的特征进行比较，初步鉴定出不同化合物类别的61种代谢物。进一步的MS/MS碎片化分析发现了古古酮E的存在，并利用MS- dial精确离子色谱工具与C. wightii阳性对照进行比对。采用固谷酮E和固谷酮Z异构体的参比标准液进行HPLC分析，验证了上述结果。另一个特征是前体离子的反diels - alder （RDA）型裂解，表明存在与乳香酸衍生物高度相似的熊烷型和齐墩烷型五环三萜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.