The synthesis of two-dimensional AuPFs/MXene nanofilm exhibiting synergistic enzymatic activity

Abstract

Two-dimensional (2D) MXenes are increasingly used in biocatalytic reactions. However, it remains a great challenge to fabricate well-defined MXenes with high catalytic activity, easy large-scale production, and high stability. Herein, for the first time, we describe a facile large-scale approach to produce a novel multi-nanozyme platform by the integration of an artificial enzyme (Au-protein self-assembly films) with 2D MXene layers (denoted as 2D MXFs) which act as biocatalysts and nanoreactors. Importantly, it is found that 2D MXFs possesses impressive biocatalytic cascade properties with both high catalytic activity and excellent stability. Specifically, the 2D MXFs exhibit a high H₂O₂ affinity, resulting in a 483- and 2.6-fold increase of catalytic activity compared with that of classical 2D MXene nanozymes and natural horseradish peroxidase, respectively. The present large-scale biomimetic strategy could represent a simple and valuable structure design and synthesis of 2D bioinspired catalytic systems to overcome the limits of 2D MXene layers for efficient biomimetic catalysis and their promising uses in different areas.