Tyramide signal amplification-based detection system: A novel approach to improve detection efficiency for circulating tumor cells

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-09-09 DOI:10.1016/j.saa.2025.126898

Lin Chen , Zhonglin Yang , Yue Lu , Shan Li , Dongjiang Tang , Lei Zhang

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Abstract

Circulating tumor cells (CTCs) are recognized as pivotal mediators in tumor metastasis, intricately linked to the onset, progression, dissemination, and persistence of cancer. Analyzing CTCs clinically can offer profound insights into patient prognosis, guide the selection of treatment regimens, assess therapeutic efficacy, and provide early warnings of recurrence and metastasis. However, the extremely low abundance of CTCs in the bloodstream poses significant challenges to traditional detection methods, resulting in low detection efficiency and high false-negative rates. To overcome this hurdle, the present study leverages the principles of tyramide signal amplification (TSA) technology to devise an efficient TSA-CTC detection system. In the simulated CTC samples with cultured cancer cells added to the blood of healthy donors, this innovative system markedly boosts the detection efficiency of TSA-CTC system, especially those exhibiting weak expression of tumor markers. In the clinical CTC samples, TSA-CTC system significantly enhances the efficiency of CTC isolation and detection (p = 0.0374) by 2.6 folds change in CTC counts of lung cancer, and 4.0 folds change of breast cancer (p = 0.0225), and 3.7 folds change of gastric cancer (p = 0.0381), and reveals notable differences (p = 0.0015) between healthy individuals and lung cancer patients. The quantities of CTCs are found to be intimately correlated with tumor size (p = 0.0452), stages (p = 0.0111), and metastasis risk (p = 0.0248). This study offers novel perspectives on the enrichment, isolation, and detection of CTCs, furnishing robust evidence and potent tools for clinical research on cancer.

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基于酪胺信号放大的检测系统：一种提高循环肿瘤细胞检测效率的新方法

循环肿瘤细胞（CTCs）被认为是肿瘤转移的关键介质，与癌症的发生、进展、传播和持续存在复杂的联系。临床分析CTCs可以深入了解患者预后，指导治疗方案的选择，评估治疗效果，并为复发和转移提供早期预警。然而，血液中ctc的丰度极低，对传统的检测方法提出了重大挑战，导致检测效率低，假阴性率高。为了克服这一障碍，本研究利用酪酰胺信号放大（TSA）技术的原理设计了一个高效的TSA- ctc检测系统。在将培养的癌细胞加入健康献血者血液的模拟CTC样本中，该创新系统显著提高了TSA-CTC系统的检测效率，特别是那些肿瘤标志物表达较弱的样本。在临床CTC样本中，TSA-CTC系统显著提高了CTC的分离和检测效率（p = 0.0374），肺癌的CTC计数变化2.6倍，乳腺癌的CTC计数变化4.0倍（p = 0.0225），胃癌的CTC计数变化3.7倍（p = 0.0381），健康个体与肺癌患者之间差异显著（p = 0.0015）。CTCs的数量与肿瘤大小（p = 0.0452）、分期（p = 0.0111）和转移风险（p = 0.0248）密切相关。本研究为CTCs的富集、分离和检测提供了新的视角，为癌症临床研究提供了有力的证据和有力的工具。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.