A magnetic-field-assisted fluorescence biosensing platform for rapid and sensitive detection of organophosphorus pesticides in food

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-09-08 DOI:10.1016/j.saa.2025.126917

Mengna Li , Rui Liu , Bang Hai , Ruobing Hao , Lei Zhao , Huan Zhan , Cuicui Li , Yanli Ma , Zhizhou Chen

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Abstract

Organophosphorus pesticides pose significant hazards to human health and can even cause fatalities, which necessitates the development of effective detection method. Herein, a magnetic-field-assisted fluorescence biosensing platform was designed for rapid and sensitive detection of organophosphorus pesticides. The underlying principle of this strategy was established upon complementary base pairing between aptamer-upconversion nanoparticles (UCNPs) and cDNA-Fe₃O₄@MoS₂. The stable stem-loop structures could provide the structural foundation enabling the aptamers to specifically recognize target pesticides. The formation of the UCNPs-aptamer-cDNA-Fe₃O₄@MoS₂ complex gradually decreased with increasing concentrations of target pesticides, leading to a reduction in fluorescence. Methyl parathion was successfully detected using this platform, achieving a detection limit of 0.002 ng/mL within a linear range of 0.01–100 ng/mL. The proposed method has also been validated in the medicinal and edible homologous foods with good recoveries. The platform could be expanded to detect other targets by using appropriate aptamers and cDNA, broadening its potential applications.

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一种快速灵敏检测食品中有机磷农药的磁场辅助荧光生物传感平台

有机磷农药对人体健康危害巨大，甚至可致人死亡，需要开发有效的检测方法。为此，设计了一种磁场辅助荧光生物传感平台，用于快速、灵敏地检测有机磷农药。该策略的基本原理是建立在适配体上转换纳米颗粒（UCNPs）和cDNA-Fe3O4@MoS2之间的互补碱基配对之上。稳定的茎环结构为适体特异性识别目标农药提供了结构基础。随着目标农药浓度的增加，UCNPs-aptamer-cDNA-Fe3O4@MoS2复合物的形成逐渐减少，导致荧光减少。在0.01 ~ 100 ng/mL的线性范围内，成功检测到甲基对硫磷，检出限为0.002 ng/mL。该方法在药用和食用同源食品中也得到了验证，回收率良好。利用合适的适体和cDNA，可以将该平台扩展到检测其他靶点，从而扩大其潜在的应用范围。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.