Bio-imprinted materials towards artificial antibodies: Technique, application and perspective

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-09-10 DOI:10.1016/j.trac.2025.118453

Lukuan Liu , Jianhui Liu , Weijie Zhang , Zhen Liang , Yukui Zhang , Jing Liu , Kaiguang Yang , Lihua Zhang

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Abstract

Bio-imprinted materials (BIMs) are artificial antibodies that use biological macromolecules as templates to create molecularly imprinted materials with precise recognition capabilities. They exhibit good physicochemical robustness under extreme conditions, making them ideal for complex biological samples. This review summarizes the fundamental principles of BIMs and the bio-imprinting technology, with a focus on their applications in various biomedical fields, including post-translational modification analysis, virus detection, extracellular vesicles and cell analysis, spatial imaging and localization, drug delivery systems, and antibody-like therapeutics. This review also discusses key technical challenges such as non-specific protein adsorption, poor batch-to-batch reproducibility, and insufficient stability in physiological conditions. Furthermore, this review provides perspectives on the use of artificial intelligence-assisted molecular design to optimize imprinting efficiency, integration strategies with existing biotechnologies, and potential future application, offering new technological pathways for the advancement of life sciences and medicine.

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面向人工抗体的生物印迹材料：技术、应用与展望

生物印迹材料是一种以生物大分子为模板制造具有精确识别能力的分子印迹材料的人工抗体。它们在极端条件下表现出良好的物理化学稳健性，使其成为复杂生物样品的理想选择。本文综述了生物印迹技术和生物印迹技术的基本原理，重点介绍了其在生物医学领域的应用，包括翻译后修饰分析、病毒检测、细胞外囊泡和细胞分析、空间成像和定位、药物传递系统和抗体样治疗等。这篇综述还讨论了关键的技术挑战，如非特异性蛋白质吸附，批间重复性差，以及生理条件下稳定性不足。此外，本文还对人工智能辅助分子设计优化印迹效率、与现有生物技术的整合策略以及未来的应用前景进行了展望，为生命科学和医学的发展提供了新的技术途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.