Investigating the cardioprotective effects of Nigella sativa oil on chronic antibiotic use on the myocardium of the left ventricle of male BALB/C mice

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-12 DOI:10.1016/j.bbrep.2025.102259

S. Saka Olusola , A. Komolafe Omobola , A. Enye Linus , A. Saka Alice , S. Ayegbusi Olateju , E. Arayombo Babatunde , Lauretta A. Frederick

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Abstract

The abuse of antibiotics is a significant factor in the emergence of antibiotic resistance and the development of several diseases in the modern world. These were conducted to provide information on the combined impact of chronic antibiotic exposure and Nigella sativa oil (NSO) on the morphology of the left ventricle of the heart of mice.

40 adult male BALB/c mice weighing about 28–32 kg, divided into 5 groups, were used for this research. Group A was given normal saline, and Group B mice received 1000 mg/kg/of ampicillin. Groups C mice received 2 ml/kg of NSO, Group D were given 1000 mg/kg of ampicillin + 2 ml/kg of NSO, and Group E mice received 1000 mg/kg pre-exposed to ampicillin + 2 ml/kg of NSO. The rats were sacrificed, and the heart tissue was harvested and stored in sample bottles containing 10 % neutral buffer for histological and immunohistochemical studies.

The results showed that there was a significant difference in superoxide dismutase and catalase activity in group B when compared with the control group (p = 0.0002, p = 0.0047, respectively). The photomicrograph of the control mice showed a regular arrangement with clear striations of myocardial fibres and centrally placed nuclei. There was a distortion of the regular arrangement of the myofibrils and disoriented nuclei in the ampicillin-only group.

The study concluded that chronic ampicillin exposure induces significant myocardial damage, affecting both the histological integrity and functional capacity of the heart.

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研究黑草油对慢性抗生素使用对雄性BALB/C小鼠左心室心肌的保护作用

抗生素的滥用是现代世界出现抗生素耐药性和几种疾病发展的一个重要因素。这些研究是为了提供慢性抗生素暴露和黑草油（NSO）对小鼠心脏左心室形态的联合影响的信息。选取体重28 ~ 32 kg的成年雄性BALB/c小鼠40只，分为5组。A组小鼠给予生理盐水，B组小鼠给予氨苄西林1000 mg/kg/。C组小鼠给予2 ml/kg NSO， D组小鼠给予1000 mg/kg氨苄西林+ 2 ml/kg NSO， E组小鼠给予1000 mg/kg氨苄西林+ 2 ml/kg NSO预暴露。处死大鼠，采集心脏组织，保存在含有10%中性缓冲液的样品瓶中，用于组织学和免疫组织化学研究。结果表明，B组的超氧化物歧化酶和过氧化氢酶活性与对照组相比差异显著（p = 0.0002, p = 0.0047）。对照组小鼠的显微照片显示心肌纤维排列规则，条纹清晰，细胞核位于中心。单用氨苄西林组肌原纤维的规则排列扭曲，细胞核定向紊乱。该研究得出结论，慢性氨苄西林暴露可引起明显的心肌损伤，影响心脏的组织学完整性和功能能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.