Joint profiling of chromatin accessibility and CRISPR edits via double-stranded DNA deaminases

IF 32.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2025-09-11 DOI:10.1038/s41592-025-02812-1

引用次数: 0

Abstract

Targeted deaminase-accessible chromatin sequencing (TDAC-seq) measures chromatin accessibility across long chromatin fibers at targeted loci using double-stranded DNA cytidine deaminases. When combined with pooled CRISPR mutational screening, TDAC-seq enables the high-throughput detection of changes in chromatin accessibility following CRISPR perturbations, allowing fine mapping of sequence–function relationships within endogenous cis-regulatory elements.

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通过双链DNA脱氨酶对染色质可及性和CRISPR编辑进行联合分析。

靶向脱氨酶可及染色质测序（TDAC-seq）使用双链DNA胞苷脱氨酶测量目标位点上长染色质纤维上的染色质可及性。当与汇集的CRISPR突变筛选相结合时，TDAC-seq能够高通量检测CRISPR扰动后染色质可及性的变化，从而可以在内源性顺式调控元件中精细绘制序列-功能关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.