Genetic linkage map construction and QTL mapping for panicle-related traits in foxtail millet (Setaria Italica (L.) P. Beauv.).

IF 3 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-09-09 eCollection Date: 2025-09-01 DOI:10.1007/s11032-025-01594-w

Huan Li, Qianxue Zhu, Tianguo Wang, Yuqin Zhao, Jiahui Fan, Lejie Yang, Shiyuan Wang, Yixuan Ma, Lu Chen, Xiaorui Li, Shuqi Dong, Xiaoqian Chu, Xiangyang Yuan, Jiagang Wang, Lulu Gao, Guanghui Yang

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引用次数: 0

Abstract

Panicle-related traits are important factors affecting the final yield of foxtail millet, and it is imperative to detect their underlying genetic basis. In this study, we constructed a genetic linkage map using the F₂ population crossed by Changsheng07 and Donggu218, two foxtail millet lines with significant differences in panicle-related traits. The genetic linkage map included 159 SSR markers and 37 Indel markers, covering 1545.5 cM, with average distance of 7.89 cM between adjacent markers. Quantitative trait loci (QTL) mapping was conducted by combining the genetic linkage map and the phenotypic data of nine panicle-related traits in F_2:3 families, and a total of 22 QTL were identified, with phenotypic variation explained (PVE) of 0.06-50.14%. Among them, the increased effect alleles of 16 QTL were derived from the parent Changsheng07, and seven QTL (qMPL3.1, qMPL5, qMPW2, qSD5, qTGW5.1, qTGW5.2 and qGL5) were major QTL, which explained the phenotypic variation of 11.41%, 12.78%, 13.75%, 34.81%, 50.14%, 40.28% and 11.32%, respectively. Moreover, 13 of 22 QTL formed four QTL clusters. These results will lay a foundation for the cloning and functional analysis of candidate genes involved in panicle development, and provide a theoretical basis for breeding high yield varieties through molecular marker-assisted selection in foxtail millet.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01594-w.

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谷子（Setaria Italica (L.)）穗部性状遗传连锁图谱构建及QTL定位p .测定)。

穗相关性状是影响谷子最终产量的重要因素，对其潜在遗传基础的研究十分必要。以穗部相关性状差异显著的两个谷子品系长胜07和东谷218杂交的F2群体为材料，构建了遗传连锁图谱。该遗传连锁图谱包含159个SSR标记和37个Indel标记，覆盖1545.5 cM，相邻标记间平均距离为7.89 cM。结合F2:3家系9个穗相关性状的遗传连锁图谱和表型数据，进行了数量性状位点（QTL）定位，共鉴定出22个QTL，表型变异解释（PVE）为0.06 ~ 50.14%。其中，16个效应增加等位基因来自亲本长胜07，其中7个QTL （qMPL3.1、qMPL5、qMPW2、qSD5、qTGW5.1、qTGW5.2和qGL5）为主要QTL，分别解释了11.41%、12.78%、13.75%、34.81%、50.14%、40.28%和11.32%的表型变异。22个QTL中有13个形成了4个QTL集群。这些结果将为谷子穗发育相关候选基因的克隆和功能分析奠定基础，并为谷子分子标记辅助选择选育高产品种提供理论依据。补充资料：在线版本包含补充资料，提供地址：10.1007/s11032-025-01594-w。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.