Chemical characterization and in vitro evaluation of the antifungal activity of Caryocar coriaceum Wittm. extracts against Candida spp. and their potential mechanisms of action

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-09 DOI:10.1016/j.micpath.2025.108031

Maria Janielly Castelo Branco Silveira , Vitória Pessoa de Farias Cabral , Daniel Sampaio Rodrigues , Lara Elloyse Almeida Moreira , Érica Rayanne Mota da Costa , Amanda Cavalcante Leitão , Thaís Lima Ferreira , Lívia Gurgel do Amaral Valente Sá , João Batista de Andrade Neto , Cecília Rocha da Silva , Manoel Odorico de Moraes , Bruno Coelho Cavalcanti , Islay Lima Magalhães , Selene Maia de Morais , Daniela Ribeiro Alves , Solange de Oliveira Pinheiro , Wildson Max Barbosa da Silva , Paulo Riceli Vasconcelos Ribeiro , Kirley Marques Canuto , Hélio Vitoriano Nobre Júnior

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引用次数: 0

Abstract

The increase in antifungal resistance requires finding alternatives to combat fungal infections, particularly those caused by species of the Candida genus. Caryocar spp., known as pequi, is a plant used in traditional medicine for its anti-inflammatory, antimicrobial and wound-healing properties. We analyzed the chemical composition and evaluated the activity of three extracts of C. coriaceum Wittm. against Candida spp. strains in both their planktonic and biofilm forms, and investigated their possible mechanisms of action. The results demonstrated antifungal activity of the leaf extracts against Candida spp. strains, with minimum inhibitory concentrations ranging from 0.5 to 512 μg/mL, although with limited effect on biofilms. Ultra-performance liquid chromatography analysis identified four main classes of compounds: flavonoids, hydrolyzable tannins, carboxylic acids, and hydroxy acids. Mechanistic analysis indicated the generation of reactive oxygen species, mitochondrial depolarization, DNA damage, and reduced cell density due to the induction of apoptosis. The extracts exhibited low cytotoxicity toward human lymphocytes. Furthermore, a synergistic effect was observed when the extracts were combined with amphotericin B, revealing a potentially synergistic association against Candida infections.

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芫荽的化学性质及体外抗真菌活性评价。抗念珠菌提取物及其潜在的作用机制。

抗真菌耐药性的增加要求找到对抗真菌感染的替代方法，特别是由念珠菌属引起的真菌感染。石竹属植物，被称为pequi，是一种传统医学中使用的植物，具有抗炎、抗菌和伤口愈合的特性。对三种提取物的化学成分进行了分析，并对其活性进行了评价。浮游形式和生物膜形式对念珠菌的抑制作用，并探讨了它们的可能作用机制。结果表明，该叶提取物对念珠菌具有一定的抑菌活性，最低抑菌浓度为0.5 ~ 512 μg/mL，但对生物膜的抑制作用有限。超高效液相色谱分析鉴定出四大类化合物：类黄酮、可水解单宁、羧酸和羟基酸。机制分析表明，由于诱导凋亡，产生活性氧，线粒体去极化，DNA损伤和细胞密度降低。提取物对人淋巴细胞具有较低的细胞毒性。此外，当提取物与两性霉素B联合使用时，观察到协同效应，揭示了对抗念珠菌感染的潜在协同关联。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)