Evaluation of the immunoprotective effect of gel 01 hydrogel inactivated vaccine against Pasteurella multocida infection in chickens

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-09 DOI:10.1016/j.micpath.2025.108027

Jiayu Chen , Yi Sun , Yujiao Hu , Jing Xia , Xinfeng Han , Yong Huang , Min Cui

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Abstract

Avian Pasteurella multocida (Pm) infection is a contagious bacterial disease causing huge economic loss in the poultry industry worldwide. Vaccination is the most widely used strategy to prevent avian Pm infection, however fowl cholera still frequently outbreaks, leading to huge economic losses to the poultry production. In this study, the genotypes of 16 strains of Pm isolated from Sichuan poultry farm between 2007 and 2022 were identified. Based on the identified genotypes of Pm, an inactivated Gel 01-adjuvanted Pm vaccine was prepared and its immunoprotective effect was comparatively assessed against aluminum hydroxide-adjuvanted, propolis-adjuvanted inactivated vaccines, and the commercial live attenuated Pm vaccine (G₁₉₀E₄₀). These results indicated that all 16 strains of avian Pm were identified as genotype A: L1. The Gel 01-adjuvanted inactivated Pm vaccine could provide 80 % protection, which is higher than that of aluminum hydroxide-adjuvanted inactivated vaccine (70 %), propolis-adjuvanted inactivated vaccine (70 %), and commercial live attenuated Pm vaccine (50 %). The Gel 01-adjuvanted inactivated Pm vaccine reduced pathological damage and provided high antibody level. The results demonstrated that A: L1 is the predominant genotype of avian Pm in the Sichuan region, and the Gel 01 adjuvant provided better immune enhancement than aluminum hydroxide and propolis adjuvant. The Gel 01 adjuvant can be used as a candidate adjuvant for avian bacterial vaccines.

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Gel 01水凝胶灭活疫苗对鸡多杀性巴氏杆菌感染的免疫保护作用评价。

禽多杀性巴氏杆菌感染是一种传染性细菌性疾病，给世界范围内的家禽业造成了巨大的经济损失。疫苗接种是预防禽Pm感染最广泛使用的策略，但禽霍乱仍然频繁暴发，给家禽生产造成巨大的经济损失。本研究对2007 - 2022年从四川家禽养殖场分离的16株Pm进行了基因型鉴定。根据鉴定的Pm基因型，制备了Gel 01佐剂Pm灭活疫苗，并与氢氧化铝佐剂、蜂胶佐剂灭活疫苗和市售Pm减毒活疫苗（G190E40）进行了免疫保护效果比较。结果表明，16株禽Pm均为A: L1基因型。凝胶01佐剂Pm灭活疫苗的保护率为80%，高于氢氧化铝佐剂灭活疫苗（70%）、蜂胶佐剂灭活疫苗（70%）和市售Pm减毒活疫苗（50%）。Gel 01佐剂的Pm灭活疫苗减少了病理性损伤，提供了高抗体水平。结果表明，A: L1是四川地区禽Pm的优势基因型，凝胶01佐剂的免疫增强效果优于氢氧化铝和蜂胶佐剂。凝胶01佐剂可作为禽细菌疫苗的候选佐剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)