Adapting a 96-well liquid culture assay to a colonial growth confirmation assay utilizing a 3D printed guide-frame system for a 96-well replicator

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-09 DOI:10.1016/j.mimet.2025.107246

Micah A. Lewis , Eric S. Adams , Elizabeth A. McMillan

引用次数: 0

Abstract

Some bacteria are not suited to assays requiring visual growth in broth; it may be useful to transfer cells from these assays to solid media to confirm growth. Here, we present a novel guide-frame system for accurate transfer of a 96-well assay to solid agar using a replicator.

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将96孔液体培养试验应用于96孔复制器的3D打印导向框架系统，以进行菌落生长确认试验。

有些细菌不适合需要在肉汤中目测生长的试验；将细胞从这些试验转移到固体培养基中以确认生长可能是有用的。在这里，我们提出了一种新的导向框架系统，用于使用复制器将96孔分析准确转移到固体琼脂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.