Assessment of Serum 3-Epi-25-Hydroxyvitamin D3, 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 in the Korean Population With UPLC–MS/MS

IF 2.9 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-09-12 DOI:10.1002/jcla.70098

Sung-Eun Cho, Jungsun Han, Gayoung Chun, Rihwa Choi, Sang Gon Lee, Hyung-Doo Park

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Abstract

Background

The liquid chromatography–tandem mass spectrometry method (LC–MS/MS) identified an epimeric form of 25-hydroxyvitamin D₃ (3-epi-25-OH-D₃) concentrations. For the first time, the 3-epi-25-OH-D₃ was included in the Korea National Health and Nutrition Examination Survey (KNHANES) IX (from 2022 to 2024). In this study, we evaluated the results of 3-epi-25-OH-D₃ in KNHANES IX to determine its clinical status in Korea.

Methods

We measured 3-epi-OH-D₃, 25-OH-D₃, and 25-OH-D₂ concentrations using an Ultra Performance (UP) LC–MS/MS, and assessed the differences in concentration ranges based on gender and age among participants in this national project.

Results

The concentration range of 3-epi-25-OH-D₃ among 11,744 Korean participants (5193 male and 6551 female, aged 10–80 with a median age of 53) was 0.50 (lower limit of quantification) to 15.66 ng/mL, with a mean ± SD of 1.15 ± 0.85 ng/mL. The 3-epi-25-OH-D₃ concentration was not significantly different between genders. The concentrations of 25-OH-D metabolites were different across age groups. There was a highly significant correlation between 3-epi-25-OH-D₃ and 25-OH-D₃ concentrations (r = 0.77, R² = 0.5911, slope = 0.0579, p < 0.0001). The minimum and maximum of % 3-epi-25-OH-D₃ were 1.33% and 23.94%, respectively. The % 3-epi-25-OH-D₃ was highest in the highest quartile (Q4) of the total 25-OH-D₃ concentration (p < 0.0001).

Conclusions

This is the first large-scale study of 3-epi-25-OH-D₃ concentrations in the Korean population. Accurate measurement of 3-epi-25-OH-D₃ is necessary for the reliable results of vitamin D metabolites in KNHANES.

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用UPLC-MS/MS评价韩国人群血清3- epi -25-羟维生素D3、25-羟维生素D3和25-羟维生素D2

背景：液相色谱-串联质谱法（LC-MS/MS）鉴定出25-羟基维生素D3 （3-epi-25-OH-D3）的外聚体浓度。3-epi-25-OH-D3首次被纳入韩国国民健康营养调查（KNHANES） IX（2022年~ 2024年）。在这项研究中，我们评估了3-epi-25-OH-D3在KNHANES IX中的结果，以确定其在韩国的临床地位。方法：我们使用超高效液相色谱-质谱联用仪测量了3-epi-OH-D3、25-OH-D3和25-OH-D2的浓度，并评估了该国家项目参与者中基于性别和年龄的浓度范围差异。结果：11,744名韩国受试者（男性5193名，女性6551名，年龄10-80岁，中位年龄53岁）3-epi-25-OH-D3浓度范围为0.50 ~ 15.66 ng/mL（定量下限），平均±SD为1.15±0.85 ng/mL。3-epi-25-OH-D3浓度在两性间无显著差异。25-OH-D代谢物浓度在不同年龄组之间存在差异。3-epi-25-OH-D3与25-OH-D3浓度呈极显著相关（r = 0.77, R2 = 0.5911，斜率= 0.0579,p = 1.33%, p = 23.94%）。在25-OH-D3总浓度的最高四分位数（Q4）中，% 3-epi-25-OH-D3最高(p结论：这是韩国人群中3-epi-25-OH-D3浓度的首次大规模研究。准确测量3-epi-25-OH-D3对于KNHANES维生素D代谢物的可靠结果是必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.