Tamlana sedimenti sp. nov. and Catalinimonas locisalis sp. nov., isolated from mangrove swamp and saltern.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-09-01 DOI:10.1099/ijsem.0.006904

Le Liu, Xiaoxuan Song, Taiyu Lin, Chuanhao Zhang, Zong-Jun Du

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Abstract

Two Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strains, designated SZ-1-7^T and 4WD22^T, were isolated from a mangrove swamp in Shenzhen and a marine solar saltern in Weihai, respectively. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SZ-1-7^T and strain 4WD22^T belong to the phylum Bacteroidota. The percentage of conserved protein and average nucleotide identity values between strain SZ-1-7^T and the genus Tamlana were 56.6-74.0% and 74.7-90.5%, respectively. Strain SZ-1-7^T grew from 15 to 40 °C (optimally at 33 °C), pH 6.0-10.0 (optimally at 7.0-8.0) and in the presence of 0-7% (w/v) NaCl (optimally in 2% NaCl). Strain SZ-1-7^T could produce carotenoid-type pigments. Strain 4WD22^T grew from 20 to 45 °C (optimally at 40 °C), pH 6.0-10.0 (optimally at 8.0) and in the presence of 1-5% (w/v) NaCl (optimally in 2% NaCl). MK-6 was the only respiratory quinone that was compatible with the genus of strain SZ-1-7^T. The strain 4WD22^T contained MK-7 as the predominant menaquinone. The major polar lipids of strain SZ-1-7^T were three kinds of unidentified lipids, five kinds of unidentified aminolipid and phosphatidylethanolamine, and those of strain 4WD22^T were phosphatidylethanolamine, three unidentified aminolipids and five unidentified lipids. The strain SZ-1-7^T was verified to have the potential to degrade polysaccharides in this study. The predatory capability of strain 4WD22^T was tested in this study. The DNA G+C contents of strain SZ-1-7^T and strain 4WD22^T were 36.5 and 41.5 mol%, respectively. Based upon the results presented in this study, strain SZ-1-7^T and strain 4WD22^T represent novel species of the genera Tamlana and Catalinimonas, respectively, for which the names Tamlana sedimenti sp. nov. and Catalinimonas locisalis sp. nov. are proposed with the type strain SZ-1-7^T (=MCCC 1H01428^T=KCTC 102108^T) and strain 4WD22^T (=MCCC 1H01431^T=KCTC 102105^T).

查看原文本刊更多论文

从红树林沼泽和盐渍化沼泽中分离的沉积褐藻属和地方卡塔利单胞菌。

从深圳红树林沼泽和威海海洋太阳能盐沼中分别分离到革兰氏阴性、需氧、非运动和棒状菌株SZ-1-7T和4WD22T。基于16S rRNA基因序列的系统发育分析表明，菌株SZ-1-7T和菌株4WD22T属于拟杆菌门。菌株SZ-1-7T与Tamlana属的保守蛋白百分比和平均核苷酸同源值分别为56.6-74.0%和74.7-90.5%。菌株SZ-1-7T在15 ~ 40°C（33°C）、pH 6.0 ~ 10.0（7.0 ~ 8.0）和0 ~ 7% (w/v) NaCl （2% NaCl）条件下生长。菌株SZ-1-7T可产生类胡萝卜素类色素。菌株4WD22T在20 - 45°C（40°C）、pH 6.0-10.0（8.0）和1-5% (w/v) NaCl （2% NaCl）条件下生长。MK-6是唯一与菌株SZ-1-7T属相容的呼吸醌。菌株4WD22T以MK-7为主。菌株SZ-1-7T的主要极性脂质为3种未识别的脂质、5种未识别的氨基脂质和磷脂酰乙醇胺，菌株4WD22T的主要极性脂质为磷脂酰乙醇胺、3种未识别的氨基脂质和5种未识别的脂质。本研究证实菌株SZ-1-7T具有降解多糖的潜力。对菌株4WD22T的捕食性进行了研究。菌株SZ-1-7T和菌株4WD22T的DNA G+C含量分别为36.5%和41.5 mol%。根据本研究结果，菌株SZ-1-7T和菌株4WD22T分别代表Tamlana属和Catalinimonas属的新种，提出菌株SZ-1-7T （=MCCC 1H01428T=KCTC 102108T）和菌株4WD22T （=MCCC 1H01431T=KCTC 102105T）的命名为Tamlana sedimenti sp. 11和catalinimalis sp. 11。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.