Eubacterium limosum as a biocatalyst for sustainable methanol bioconversion

IF 7 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology Pub Date : 2025-09-10 DOI:10.1016/j.copbio.2025.103354

Jiyun Bae , Hyunwoo Jung , Byung-Kwan Cho

引用次数: 0

Abstract

Eubacterium limosum is an acetogenic bacterium capable of utilizing one-carbon substrates — CO₂, CO, formate, and methanol — while producing valuable reduced compounds, including butyrate and hexanoate. Its broad substrate range and robust methanol utilization make it a promising platform for sustainable methanol bioconversion. Recent advances in genetic and analytical tools have enabled comprehensive systems-level characterization and metabolic engineering. Additionally, methanol mixotrophy strategies have significantly improved energy availability and product yields. This review examines current progress in E. limosum research, focusing on genetic tool development, methanol-based biochemical production, and mixotrophy implementation. We conclude by outlining future directions for enhancing strain performance and establishing E. limosum as an industrial biocatalyst for methanol valorization.

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石灰真杆菌作为可持续甲醇生物转化的生物催化剂。

石灰真杆菌是一种产丙酮的细菌，能够利用单碳底物——二氧化碳、一氧化碳、甲酸盐和甲醇——同时产生有价值的还原性化合物，包括丁酸盐和己酸盐。其广泛的底物范围和强大的甲醇利用率使其成为可持续甲醇生物转化的有前途的平台。遗传和分析工具的最新进展使全面的系统级表征和代谢工程成为可能。此外，甲醇混合发酵策略显著提高了能源利用率和产品产量。本文综述了羊粪草的研究进展，重点介绍了遗传工具的开发、基于甲醇的生化生产和混合营养的实施。最后，我们概述了未来的发展方向，以提高菌株的性能，并建立E. limosum作为甲醇增值的工业生物催化剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in biotechnology 工程技术-生化研究方法

CiteScore

16.20

自引率

2.60%

发文量

226

审稿时长

4-8 weeks

期刊介绍： Current Opinion in Biotechnology (COBIOT) is renowned for publishing authoritative, comprehensive, and systematic reviews. By offering clear and readable syntheses of current advances in biotechnology, COBIOT assists specialists in staying updated on the latest developments in the field. Expert authors annotate the most noteworthy papers from the vast array of information available today, providing readers with valuable insights and saving them time. As part of the Current Opinion and Research (CO+RE) suite of journals, COBIOT is accompanied by the open-access primary research journal, Current Research in Biotechnology (CRBIOT). Leveraging the editorial excellence, high impact, and global reach of the Current Opinion legacy, CO+RE journals ensure they are widely read resources integral to scientists' workflows. COBIOT is organized into themed sections, each reviewed once a year. These themes cover various areas of biotechnology, including analytical biotechnology, plant biotechnology, food biotechnology, energy biotechnology, environmental biotechnology, systems biology, nanobiotechnology, tissue, cell, and pathway engineering, chemical biotechnology, and pharmaceutical biotechnology.