Ian John P Alberba, Sean Maynard M Solis, Jill Mae Marie R Base, Nicole O Julia, Janelle Ranee F Villanueva, Chanie Y Patanindagat, Maureen B Sabit, John Donnie A Ramos
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引用次数: 0
Abstract
Background: Dermatophagoides pteronyssinus (Dp), with its major allergen Der p 2, triggers allergy symptoms in various populations worldwide, but their IgE-binding properties among Filipinos remain limited.
Objectives: This study investigated the IgE reactivity of Dp and its major allergen rDer p 2 in a cohort of allergic and nonallergic Filipinos from Ilocos Norte, Philippines.
Methods: Using enzyme-linked immunosorbent assay, total IgE (n = 960), Dp-specific IgE (n = 247), and rDer p 2-specific IgE (n = 90) levels were determined. The odds ratio was used to determine the association between Dp and rDer p 2 specific IgE among selected demographic variables. An inhibition enzyme-linked immunosorbent assay was used to measure the inhibition of rDer p 2 against Dp allergen extract.
Results: Among 171 allergic cases, mean IgE levels were significantly higher (P < 0.01) than in 76 nonallergic controls. Of the allergic cases, 85 individuals (49.71%) were sensitized to Dp allergens, with 68.89% of these also displaying sensitization to rDer p 2. On average, rDer p 2 inhibited IgE binding to Dp allergens by approximately 36%. Significant associations were found between Dp sensitization and age (P < 0.01), male gender (P = 0.01), and between rDer p 2-sensitization and atopy (P = 0.01).
Conclusion: This study has shown the importance of Dp extract and its major allergen rDer p 2 as a source of allergen that sensitizes atopic Filipino individuals. Consequently, the inclusion of Dp and rDer p 2 in the panel of reagents utilized in immunodiagnostic and immunotherapeutic strategies and interventions for the local Filipino population sensitized to house dust mites is highly recommended.
期刊介绍:
Asia Pacific Allergy (AP Allergy) is the official journal of the Asia Pacific Association of Allergy, Asthma and Clinical Immunology (APAAACI). Although the primary aim of the journal is to promote communication between Asia Pacific scientists who are interested in allergy, asthma, and clinical immunology including immunodeficiency, the journal is intended to be available worldwide. To enable scientists and clinicians from emerging societies appreciate the scope and intent of the journal, early issues will contain more educational review material. For better communication and understanding, it will include rational concepts related to the diagnosis and management of asthma and other immunological conditions. Over time, the journal will increase the number of original research papers to become the foremost citation journal for allergy and clinical immunology information of the Asia Pacific in the future.