Laura Bellec, Célia Le Maire, Nathalie Marnet, Chrystelle Paty, Anne-Marie Cortesero, Maxime R Hervé
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引用次数: 0
Abstract
Plants defend themselves against herbivorous insects through diverse morphological and biochemical traits. Non-protein amino acids (NPAAs) are an important component of the plant metabolome, although their defensive function remains largely unexplored. Here, we investigated the role of S-methyl-l-cysteine sulfoxide (SMCSO), a sulphur-containing NPAA accumulated in Brassicaceae, in mediating plant defence against herbivorous insects. SMCSO was quantified in inflorescences, leaves (young and old) and roots (primary and secondary) of 14 Brassicaceae species. Additionally, feeding tests on artificial substrates supplemented with physiological SMCSO levels were conducted with both generalist and specialist herbivorous insects feeding on one of the different plant parts studied. In line with the optimal defence theory, we found higher SMCSO levels in reproductive than vegetative tissues, and in young leaves and primary roots compared to old leaves and secondary roots, respectively. SMCSO also exerted a consistent phagodeterrent effect on generalist herbivores, while specialists showed variable responses from deterrence to stimulation. This study provides the first evidence of the influence of this compound on herbivore feeding behaviour, with effects depending on the diet breadth. These findings broaden our understanding of plant chemical defence complexity and highlight the ecological role of NPAAs in plant-insect interactions.
植物通过不同的形态和生化特征来保护自己免受食草昆虫的侵害。非蛋白氨基酸(NPAAs)是植物代谢组的重要组成部分,尽管其防御功能在很大程度上尚未被研究。本文研究了十字花科植物积累的含硫NPAA s -甲基-l-半胱氨酸亚砜(SMCSO)在植物防御食草昆虫中的作用。对14种芸苔科植物的花序、叶片(幼叶和老叶)和根(主次根)的SMCSO进行了定量分析。此外,在添加了生理SMCSO水平的人工基质上进行了取食试验,让通用型和专一型草食性昆虫取食所研究的不同植物部分之一。根据最优防御理论,我们发现SMCSO在生殖组织中的含量高于营养组织,在幼叶和初生根中的含量分别高于老叶和次生根。SMCSO对一般食草动物也有一致的吞噬威慑作用,而专家则表现出从威慑到刺激的不同反应。这项研究首次证明了这种化合物对草食动物摄食行为的影响,其影响取决于饮食的广度。这些发现拓宽了我们对植物化学防御复杂性的理解,并突出了NPAAs在植物-昆虫相互作用中的生态作用。
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.