{"title":"Genetic Regulation Differences of VRS Genes in the Development of Lateral Spikelets in Two-Rowed Barley.","authors":"Liping Shen, Zhiwen Sun, Yangyang Liu, Kuocheng Shen, Zhimin Wang, Botao Ye, Ziying Wang, Zifeng Guo","doi":"10.1111/pce.70183","DOIUrl":null,"url":null,"abstract":"<p><p>The barley (Hordeum vulgare L.) spike consists of one central and two lateral spikelets at each rachis node. In two-rowed barley, only the central spikelet is fertile, the lateral spikelets also produce grain while in six-rowed barley. Five SIX-ROWED SPIKE genes (VRS1-5) have been identified as regulators of lateral spikelet fertility in barley, but the underlying genetic mechanisms of these VRS genes remain unclear. In this study, we conducted a detailed observation of the development process of the lateral spikelets in two-rowed barley and performed comparative transcriptome analysis to investigate gene expression differences between vrs1-5 mutants and wild-type spikelets. This revealed the differences in the downstream pathways regulated by the VRS genes in lateral spikelet development and the correlation of the effects of different VRS genes on lateral spikelet fertility. Using chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq), we identified 213 direct downstream genes of VRS1, including those involved in energy metabolism, hormone pathways, and transcription factors. We also discovered that VRS1 directly binds to the D-class gene HvMADS13 to regulate spikelet fertility. Further analysis of the six-rowed barley accessions revealed that a 1 bp deletion in the C-terminus of VRS1 disrupts its ability to repress transcription, leading to fertility in the lateral spikelets.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.70183","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The barley (Hordeum vulgare L.) spike consists of one central and two lateral spikelets at each rachis node. In two-rowed barley, only the central spikelet is fertile, the lateral spikelets also produce grain while in six-rowed barley. Five SIX-ROWED SPIKE genes (VRS1-5) have been identified as regulators of lateral spikelet fertility in barley, but the underlying genetic mechanisms of these VRS genes remain unclear. In this study, we conducted a detailed observation of the development process of the lateral spikelets in two-rowed barley and performed comparative transcriptome analysis to investigate gene expression differences between vrs1-5 mutants and wild-type spikelets. This revealed the differences in the downstream pathways regulated by the VRS genes in lateral spikelet development and the correlation of the effects of different VRS genes on lateral spikelet fertility. Using chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq), we identified 213 direct downstream genes of VRS1, including those involved in energy metabolism, hormone pathways, and transcription factors. We also discovered that VRS1 directly binds to the D-class gene HvMADS13 to regulate spikelet fertility. Further analysis of the six-rowed barley accessions revealed that a 1 bp deletion in the C-terminus of VRS1 disrupts its ability to repress transcription, leading to fertility in the lateral spikelets.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.