DNA Damage Markers, Thioredoxin System, and Inflammation in a Population Exposed to High Indoor Radon Levels.

IF 2.7 3区 医学 Q2 BIOLOGY
Dwi Ramadhani, Sofiati Purnami, Tiara Andalya Oktariyani, Dira Syafira, Viria Agesti Suvifan, Iin Kurnia Hasan Basri, Teja Kisnanto, Heri Wibowo, Masaru Yamaguchi, Ikuo Kashiwakura, Tomisato Miura, Mukh Syaifudin, Septelia Inawati Wanandi, Retno Widowati
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Abstract

High radon levels in the environment can lead to adverse biological effects such as DNA damage, thereby increasing cancer risk, especially lung cancer. This study focused on Tande-Tande sub-village in Mamuju, West Sulawesi, Indonesia, an area known for naturally high indoor radon concentrations, where inhabitants have been chronically exposed to radon throughout their lives. Blood samples from 38 subjects in Tande-Tande sub-village and the control area, Topoyo village, were examined. We then evaluated the DNA damage by assessing γ-H2AX for double-strand breaks and 8-hydroxydeoxyguanosine (8-OHdG) for oxidative damage. Additionally, we measured key molecules of the thioredoxin (Trx) system, Trx and thioredoxin reductase (TrxR), to gauge antioxidant levels and thus, oxidative stress response status. The C-reactive protein (CRP) to albumin ratio was analyzed to assess inflammatory status. Comparison of 8-OHdG, Trx, TrxR concentrations, and CRP/Albumin between the exposed and control groups were assessed by unpaired Student's t-test or Mann-Whitney test, depending on the normality of the data distribution. Correlations between concentration of 8-OHdG, Trx, TrxR or CRP/Albumin and indoor radon concentrations were investigated using either the Pearson or Spearman correlation tests, based on the distribution characteristics of the data. Our analyses of DNA damage markers (γ-H2AX and 8-OHdG), Trx, TrxR, and CRP/albumin ratio showed no significant increase in DNA damage markers in Tande-Tande sub-village residents compared to controls. Levels of 8-OHdG, Trx, and TrxR were significantly lower in Tande-Tande sub-village inhabitants when compared to the control area (P < 0.0001, P = 0.002, and P = 0.003, respectively), whereas CRP/albumin ratio did not differ significantly between these groups (P = 0.844). The present study did not find significant evidence of increased DNA damage, antioxidant system activity, or inflammatory status in inhabitants exposed to high radon levels. There is a possibility that the excessive ROS production existed in the early life period and subsequently manifested as a radio-adaptive response (RAR) during the adulthood of this population. These findings also support our previous assumptions that the excessive ROS production disrupts redox signaling and leads to a reduction in antioxidant levels.

DNA损伤标记、硫氧还蛋白系统和暴露于高室内氡水平人群的炎症。
环境中的高氡水平可导致不利的生物效应,如DNA损伤,从而增加癌症风险,特别是肺癌风险。本研究的重点是印度尼西亚西苏拉威西岛Mamuju的Tande-Tande村,该地区以室内氡自然浓度高而闻名,居民一生长期暴露于氡。对来自Tande-Tande村和对照区Topoyo村的38名受试者进行血样检测。然后,我们通过评估γ-H2AX双链断裂和8-羟基脱氧鸟苷(8-OHdG)氧化损伤来评估DNA损伤。此外,我们测量了硫氧还蛋白(Trx)系统的关键分子,Trx和硫氧还蛋白还原酶(TrxR),以测量抗氧化水平,从而测量氧化应激反应状态。分析c反应蛋白(CRP)与白蛋白的比值以评估炎症状态。根据数据分布的正态性,采用非配对学生t检验或Mann-Whitney检验评估暴露组和对照组之间8-OHdG、Trx、TrxR浓度和CRP/白蛋白的比较。根据数据的分布特征,采用Pearson或Spearman相关检验,研究8-OHdG、Trx、TrxR或CRP/白蛋白浓度与室内氡浓度的相关性。我们对DNA损伤标志物(γ-H2AX和8-OHdG)、Trx、TrxR和CRP/白蛋白比率的分析显示,与对照组相比,坦德-坦德村居民的DNA损伤标志物没有显著增加。与对照组相比,坦德-坦德村居民的8-OHdG、Trx和TrxR水平显著降低(分别为P < 0.0001、P = 0.002和P = 0.003),而CRP/白蛋白比值在两组之间无显著差异(P = 0.844)。本研究未发现暴露于高氡水平的居民DNA损伤、抗氧化系统活性或炎症状态增加的显著证据。有一种可能性是,过量的ROS产生存在于生命早期,随后在该人群的成年期表现为辐射适应性反应(RAR)。这些发现也支持了我们之前的假设,即过量的ROS产生会破坏氧化还原信号并导致抗氧化剂水平降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Radiation research
Radiation research 医学-核医学
CiteScore
5.10
自引率
8.80%
发文量
179
审稿时长
1 months
期刊介绍: Radiation Research publishes original articles dealing with radiation effects and related subjects in the areas of physics, chemistry, biology and medicine, including epidemiology and translational research. The term radiation is used in its broadest sense and includes specifically ionizing radiation and ultraviolet, visible and infrared light as well as microwaves, ultrasound and heat. Effects may be physical, chemical or biological. Related subjects include (but are not limited to) dosimetry methods and instrumentation, isotope techniques and studies with chemical agents contributing to the understanding of radiation effects.
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