Nitrifying Communities in Biological Nitrogen Removal Processes at Tropical Municipal Wastewater Treatment Plants.

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2025-01-01 DOI:10.1264/jsme2.ME25036

Liang Feng, Jia Xing Loi, Joana Séneca, Petra Pjevac, Faidzul Hakim Adnan, Gek Cheng Ngoh, Bee Chin Khor, Alijah Mohd Aris, Mamoru Oshiki, Holger Daims, Adeline Seak May Chua

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Abstract

Nitrifying communities in activated sludge play a crucial role in biological nitrogen removal processes in municipal wastewater treatment plants. While extensive research has been conducted in temperate regions, limited information is available on nitrifiers in tropical regions. The present study investigated all currently known nitrifying communities in two full-scale municipal wastewater treatment plants in Malaysia operated under low-dissolved oxygen (DO) (0.2-0.7‍ ‍mg‍ ‍DO‍ ‍L^-1) or high-DO (2.0-5.5‍ ‍mg‍ ‍DO‍ ‍L^-1) conditions at 30°C. The core nitrifiers in the municipal wastewater treatment plants were Nitrosomonas (ammonia-oxidizing bacteria, AOB), Nitrospira (nitrite-oxidizing or complete ammonia-oxidizing, comammox, bacteria), and ammonia-oxidizing archaea (AOA) as identified by a 16S rRNA gene amplicon sequencing ana-lysis and corroborated by 16S rRNA-targeted fluorescence in situ hybridization. A quantitative polymerase chain reaction (qPCR) targeting ammonia monooxygenase subunit A (amoA) genes revealed stable populations of comammox Nitrospira and AOB in both wastewater treatment plants. AOA were detected in only one of the plants and their population sizes fluctuated, with higher temporary abundance under high-DO conditions. These results provide important insights into the composition and dynamics of nitrifying communities in tropical municipal wastewater treatment plants.

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热带城市污水处理厂生物脱氮过程中的硝化群落。

活性污泥中的硝化群落在城市污水处理厂的生物脱氮过程中起着至关重要的作用。虽然在温带地区进行了广泛的研究，但关于热带地区硝化菌的信息有限。本研究调查了马来西亚两个大型城市污水处理厂中目前已知的所有硝化群落，这些污水处理厂在30°C的低溶解氧（DO）（0.2-0.7 mg）或高DO （2.0-5.5 mg）条件下运行。16S rRNA基因扩增子测序分析和16S rRNA靶向荧光原位杂交证实，城市污水处理厂的核心硝化菌为亚硝化单胞菌（氨氧化细菌，AOB）、硝化螺旋菌（亚硝酸盐氧化或完全氨氧化细菌，comammox，细菌）和氨氧化古菌（AOA）。利用针对氨单加氧酶亚基A （amoA）基因的定量聚合酶链反应（qPCR）揭示了两个污水处理厂中硝螺旋菌和AOB的稳定种群。AOA仅在1种植物中检测到，种群大小波动，在高do条件下暂时丰度较高。这些结果为热带城市污水处理厂硝化群落的组成和动态提供了重要的见解。

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.