Expansion and revision of the genus Xanthobacter and proposal of Roseixanthobacter gen. nov.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-09-01 DOI:10.1099/ijsem.0.006909

Maximillian P M Soltysiak, Amogh P Jalihal, Caroline E Christophersen, Audrey L H Ory, Andrew D Lee, Jessica Boulton, Michael Springer

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引用次数: 0

Abstract

The nitrogen-fixing, chemolithoautotrophic genus Xanthobacter is found across numerous diverse environments worldwide and is an important member of many ecosystems. These species serve as model systems for their metabolic properties and have industrial applications in bioremediation and sustainable protein, food and fertilizer production. Despite their abundance and utility, the majority of Xanthobacter strains are without a genome sequence, and only eight validly published species are known to date. To expand our understanding of the diversity and evolutionary history of the genus, we sequenced the genomes of 37 repository strains and 26 novel environmental strains we isolated. After performing comparative phylogenomic analyses, we expand and revise the genus Xanthobacter and propose the novel genus Roseixanthobacter gen. nov. For Xanthobacter, we describe 9 novel species (bringing the total to 17): Xanthobacter albus sp. nov. (V0C-6^T=DSM 117699^T=ATCC TSD-450^T), Xanthobacter cornucopiae sp. nov. (V4C-4^T=DSM 117704^T=ATCC TSD-451^T), Xanthobacter lutulentifluminis sp. nov. (V3C-3^T=DSM 117706^T=ATCC TSD-452^T), Xanthobacter nonsaccharivorans sp. nov. (14g^T=DSM 431^T=JCM 1201^T=CIP 105432^T=NCIMB 10811^T), Xanthobacter pseudotagetidis sp. nov. (KA^T=DSM 11602^T=KCTC 8467^T), Xanthobacter sediminis sp. nov. (V8C-5^T=DSM 117708^T=ATCC TSD-453^T), Xanthobacter variabilis sp. nov. (V4C-8^T=DSM 117713^T=ATCC TSD-454^T), Xanthobacter versatilis sp. nov. (Py2^T=DSM 118458^T=ATCC BAA-1158^T) and Xanthobacter wiegelii sp. nov. (RH 10^T=DSM 597^T=JCM 7864^T=CIP 105437^T). For Roseixanthobacter gen. nov., we describe five novel species formerly classified as Xanthobacter: Roseixanthobacter finlandensis sp. nov. (VTT E-85241^T=BIO GISA 20^T=DSM 117884^T), Roseixanthobacter glucoisosaccharinicivorans sp. nov. (VTT E-85242^T=BIO GISA 21^T=DSM 117885^T), Roseixanthobacter liquoris sp. nov. (VTT E-85238^T=BIO GISA 16^T=DSM 117882^T), Roseixanthobacter pseudopolyaromaticivorans sp. nov. (VTT E-85240^T=BIO GISA 19^T=DSM 117883^T) and Roseixanthobacter psychrophilus sp. nov. (W30^T=DSM 24535^T=KCTC 8466^T). We characterized the phenotypic properties of these type strains, including temperature, salinity and pH ranges, carbon substrate utilization, motility, slime production, autotrophic growth and enzymatic activities. We discovered a more diverse range of phenotypes across the genus Xanthobacter than previously known and elucidated the evolutionary history within the genus. These findings and genome sequences will help further the application of Xanthobacter biology in academic, industrial and environmental settings and provide additional insight into the unique biological properties that make these species attractive for such applications.

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黄杆菌属的扩展与修正及玫瑰黄杆菌属的提出。

固氮、化能自养的黄杆菌属（Xanthobacter）在世界上许多不同的环境中都有发现，是许多生态系统的重要成员。这些物种作为其代谢特性的模型系统，在生物修复和可持续蛋白质、食品和肥料生产中具有工业应用。尽管黄杆菌数量众多，用途广泛，但大多数黄杆菌菌株没有基因组序列，迄今为止已知的有效发表的物种只有8种。为了扩大我们对该属的多样性和进化史的了解，我们对37个储存库菌株和26个新分离的环境菌株进行了基因组测序。在进行系统基因组比较分析后，我们对黄杆菌属进行了扩展和修正，并提出了新属Roseixanthobacter gen. 11 .对于黄杆菌属，我们描述了9个新种（使总数达到17个）：黄杆菌（V0C-6T=DSM 117699T=ATCC TSD-450T）、玉米黄杆菌（V4C-4T=DSM 117704T=ATCC TSD-451T）、lutulentifluminis黄杆菌（V3C-3T=DSM 117706T=ATCC TSD-452T）、非糖化黄杆菌（14gT=DSM 431T=JCM 1201T=CIP 105432T=NCIMB 10811T）、伪tagetitis黄杆菌（KAT=DSM 11602T=KCTC 8467T）、sediminis黄杆菌（V8C-5T=DSM 117708T=ATCC TSD-453T）变异黄杆菌（V4C-8T=DSM 117713T=ATCC TSD-454T），多面黄杆菌（Py2T=DSM 118458T=ATCC BAA-1158T）和威氏黄杆菌（RH 10T=DSM 597T=JCM 7864T=CIP 105437T）。对于Roseixanthobacter gen. nov.，我们描述了五个以前被归类为黄杆菌的新种：芬兰玫瑰花菌（VTT E-85241T=BIO GISA 20T=DSM 117884T）、糖异糖玫瑰花菌（VTT E-85242T=BIO GISA 21T=DSM 117885T）、酒玫瑰花菌（VTT E-85238T=BIO GISA 16T=DSM 117882T）、伪多芳香玫瑰花菌（VTT E-85240T=BIO GISA 19T=DSM 117883T）和嗜寒玫瑰花菌（W30T=DSM 24535T=KCTC 8466T）。我们对这些类型菌株的表型特性进行了表征，包括温度、盐度和pH范围、碳底物利用、运动、粘液生成、自养生长和酶活性。我们发现了比以前已知的更多样化的表型范围，并阐明了属内的进化史。这些发现和基因组序列将有助于进一步在学术、工业和环境环境中应用黄杆菌生物学，并为这些物种独特的生物学特性提供更多的见解，这些特性使这些物种对这些应用具有吸引力。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.