Free-living amoebae population in a caldera lake after a phreatomagmatic eruption

IF 2.5 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-09-08 DOI:10.1016/j.actatropica.2025.107830

Sofia Jade Wong , Kyle Christian Cabalquinto , Caitrin Arelle Castillo , Alexis Dominique Chua , Juliana Lizbeth Dayao , Izza Mae De Ocampo , Alec Jan Delos Reyes , Frederick Ramirez Masangkay , Giovanni De Jesus Milanez

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引用次数: 0

Abstract

Volcanic eruptions are natural disasters that can change life and the ecosystem. Through molecular testing, this study identified the free-living amoebae (FLA) population in a caldera lake following a phreatomagmatic eruption in 2020 and compared it with pre-established data in 2016 in the same lake. Four sampling sites were identified using purposively sampling, namely: Laurel (T1), Agoncillo (T2), San Nicolas (T3), and Talisay (T4). Water samples were filtered, cultured on NNA lawned with Escherichia coli, and checked microscopically for 14 days. Twelve (12; 30 %) of 40 water samples yielded positive amoebic growth from the water samples. Site T4 had the highest growth. Polymerase chain reaction and sequencing confirmed the presence of Acanthamoeba T4 genotype in two isolates (PP741735 and PP741736). Volcanic eruptions can potentially disrupt the biodiversity of FLA and promote the proliferation of pathogenic forms. This poses health risks to tourists who frequent the area and, more importantly, residents residing along its shores. Local government involvement is necessary in cautioning residents, especially those who have returned from evacuation centers, about using the caldera waters, which is paramount to mitigating future cases.

查看原文本刊更多论文

自由生活的阿米巴种群在火山口湖后的一个呼吸岩浆爆发。

火山爆发是可以改变生命和生态系统的自然灾害。通过分子测试，本研究确定了2020年一次呼吸岩浆喷发后火山口湖中自由生活的阿米巴（FLA）种群，并将其与2016年同一湖泊的预建立数据进行了比较。通过有目的取样确定了4个采样点，分别是：Laurel （T1）、Agoncillo （T2）、San Nicolas （T3）和Talisay （T4）。过滤水样，在带有大肠杆菌的NNA上培养，显微镜下观察14天。40个水样中有12个（12.30%）的水样产生了正的阿米巴生长。T4站点生长最快。聚合酶链反应和测序证实两株分离株（PP741735和PP741736）存在棘阿米巴T4基因型。火山爆发可能会破坏FLA的生物多样性，并促进致病性形式的增殖。这对经常光顾该地区的游客，更重要的是，对居住在沿岸的居民构成了健康风险。在提醒居民，特别是那些从疏散中心返回的居民，不要使用火山口的水时，地方政府的参与是必要的，这对减少未来的情况至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.