How Real-Time Kinetics Explain the Different Interaction of Cubosomes and Hexosomes Nanoparticles with Lipid Membranes

IF 4.4 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Mario Campana, Silvia Dante, Paola Astolfi, Simone Ranieri, Michela Pisani
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Abstract

Cubosomes and hexosomes are interesting structures with diverse applications as drug and gene delivery systems, as well as theranostics. Understanding the uptake mechanism of these nanostructures by cell membranes is fundamental to improving and broadening their application. In this study, the kinetics of interaction of monoolein (GMO) cubosomes or dioleoylphosphatidylethanolamine (DOPE) hexosomes with supported lipid bilayers (SLBs) with varying compositions and stiffness, as valuable model membranes, are investigated by neutron reflectivity (NR) coupled with quartz crystal microbalance with dissipation monitoring (QCM-D). Atomic force microscopy (AFM) is also used to analyze SLBs topography after such interaction. Cubosomes rapidly interact with SLBs, resulting in lipid exchange between the two systems, destabilizing the bilayer, and ultimately causing its removal. The inclusion of cholesterol (CHOL) in the SLB confers rigidity, making it more resistant to removal. Completely different results are obtained when hexosomes interact with SLBs: an intrinsic resistance of the bilayers toward these nanoparticles is observed, regardless of CHOL presence, combined with a slight change in SLB composition, suggesting that hexosomes tend to fuse with the SLB. These findings provide new and valuable insights into the potential mechanisms and kinetics of cellular uptake of these systems, enhancing their potential as drug delivery vectors.

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实时动力学如何解释立方体和六体纳米颗粒与脂质膜的不同相互作用
立方体体和自体体是一种有趣的结构,在药物和基因传递系统以及治疗学方面有着广泛的应用。了解这些纳米结构被细胞膜吸收的机制是改善和扩大其应用的基础。本研究采用中子反射率(NR)与石英晶体微平衡耗散监测(QCM-D)相结合的方法,研究了单油素(GMO)立方体体或二油基磷脂酰乙醇胺(DOPE)自旋体与不同组成和刚度的负载脂质双层(slb)作为有价值的模型膜的相互作用动力学。原子力显微镜(AFM)也用于分析这种相互作用后slb的形貌。立方体体迅速与slb相互作用,导致两个系统之间的脂质交换,破坏双分子层的稳定,并最终导致其去除。SLB中包含胆固醇(CHOL)赋予其刚性,使其更不易清除。当自体体与SLB相互作用时,得到了完全不同的结果:观察到双层对这些纳米粒子的内在抵抗力,无论CHOL是否存在,再加上SLB成分的轻微变化,表明自体体倾向于与SLB融合。这些发现为细胞摄取这些系统的潜在机制和动力学提供了新的和有价值的见解,增强了它们作为药物传递载体的潜力。
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来源期刊
Advanced Materials Interfaces
Advanced Materials Interfaces CHEMISTRY, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
8.40
自引率
5.60%
发文量
1174
审稿时长
1.3 months
期刊介绍: Advanced Materials Interfaces publishes top-level research on interface technologies and effects. Considering any interface formed between solids, liquids, and gases, the journal ensures an interdisciplinary blend of physics, chemistry, materials science, and life sciences. Advanced Materials Interfaces was launched in 2014 and received an Impact Factor of 4.834 in 2018. The scope of Advanced Materials Interfaces is dedicated to interfaces and surfaces that play an essential role in virtually all materials and devices. Physics, chemistry, materials science and life sciences blend to encourage new, cross-pollinating ideas, which will drive forward our understanding of the processes at the interface. Advanced Materials Interfaces covers all topics in interface-related research: Oil / water separation, Applications of nanostructured materials, 2D materials and heterostructures, Surfaces and interfaces in organic electronic devices, Catalysis and membranes, Self-assembly and nanopatterned surfaces, Composite and coating materials, Biointerfaces for technical and medical applications. Advanced Materials Interfaces provides a forum for topics on surface and interface science with a wide choice of formats: Reviews, Full Papers, and Communications, as well as Progress Reports and Research News.
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