Selective bactericidal efficacy of 465-nm blue light phototherapy against standard and canine wound pathogens: An in vitro evaluation.

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-07-01 Epub Date: 2025-07-27 DOI:10.14202/vetworld.2025.2064-2071
Pimsiri Ngowwatana, Naruepon Kampa, Somphong Hoisang, Suvaluk Seesupa, Duangdaow Khunbutsri, Saikam Chaimongkol, Preenun Jitasombuti, Supranee Jitpean, Thanikul Srithunyarat, Chalermkwan Nonthakotr, Nitaya Boonbal, Piyasak Wipoosak, Duangdaun Kaenkangploo
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引用次数: 0

Abstract

Background and aim: Antibiotic resistance poses a growing threat to wound management in veterinary medicine. Blue light phototherapy has emerged as a non-antibiotic bactericidal alternative with additional benefits for wound healing. However, its effectiveness in clinical veterinary contexts remains inadequately explored. This study evaluated the bactericidal efficacy of 465-nm blue light against standard pathogens and bacteria isolated from infected canine wounds, aiming to determine optimal energy doses for clinical use.

Materials and methods: Three standard bacterial strains - Staphylococcus aureus (American Type Culture Collection [ATCC] 25923), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922) - along with five clinical isolates from canine wounds, were exposed to 465-nm blue light at energy doses of 28, 56, and 112 J/cm2 (15, 30, and 60 min, respectively). Colony-forming units (CFUs) were quantified post-irradiation and compared to non-irradiated controls. Statistical significance was assessed using appropriate parametric and non-parametric tests.

Results: P. aeruginosa (ATCC 27853) exhibited significant, dose-dependent inhibition at all energy doses, resulting in reductions of 36.3%, 60.5%, and 82.8%. Clinical P. aeruginosa isolates demonstrated 21.1% and 78.8% inhibition at 56 and 112 J/cm2, respectively (p < 0.05). E. coli (ATCC 25922) was significantly inhibited only at 112 J/cm2 (46.4% reduction, p = 0.045). No significant reductions were observed for S. aureus, Acinetobacter baumannii, Staphylococcus haemolyticus, clinical E. coli, or Enterococcus faecalis at any dose.

Conclusion: Blue light at 465-nm exhibits selective bactericidal activity, effectively inhibiting P. aeruginosa and E. coli (ATCC), with efficacy dependent on bacterial species and applied energy dose. Its limited effect on other pathogens underscores the importance of species-specific treatment planning. Higher energy doses (112 J/cm2) may be required in unknown or mixed infections. Further investigation is recommended to refine device specifications and assess clinical utility in veterinary settings.

Abstract Image

465纳米蓝光光疗对标准和犬伤口病原菌的选择性杀菌效果:体外评价。
背景与目的:抗生素耐药性对兽医伤口管理构成越来越大的威胁。蓝光光疗已成为一种非抗生素杀菌替代,对伤口愈合有额外的好处。然而,其在临床兽医环境中的有效性仍未得到充分探讨。本研究评估了465纳米蓝光对从感染犬伤口中分离的标准病原体和细菌的杀菌效果,旨在确定临床使用的最佳能量剂量。材料和方法:三种标准菌株-金黄色葡萄球菌(美国型培养收集[ATCC] 25923),铜绿假单胞菌(ATCC 27853)和大肠杆菌(ATCC 25922) -以及犬伤口的五种临床分离株,分别暴露在能量剂量为28,56和112 J/cm2的465 nm蓝光下(分别为15,30和60分钟)。对辐照后的菌落形成单位(CFUs)进行量化,并与未辐照对照进行比较。采用适当的参数检验和非参数检验评估统计显著性。结果:P. aeruginosa (ATCC 27853)在所有能量剂量下均表现出显著的剂量依赖性抑制作用,分别降低36.3%、60.5%和82.8%。临床分离的铜绿假单胞菌在56 J/cm2和112 J/cm2时的抑菌率分别为21.1%和78.8% (p < 0.05)。大肠杆菌(ATCC 25922)仅在112 J/cm2时被显著抑制(减少46.4%,p = 0.045)。在任何剂量下,均未观察到金黄色葡萄球菌、鲍曼不动杆菌、溶血葡萄球菌、临床大肠杆菌或粪肠球菌的显著减少。结论:465 nm蓝光具有选择性杀菌活性,可有效抑制P. aeruginosa和E. coli (ATCC),且效果与细菌种类和施加能量剂量有关。它对其他病原体的作用有限,强调了针对特定物种制定治疗计划的重要性。未知感染或混合性感染可能需要更高的能量剂量(112焦耳/平方厘米)。建议进一步调查以完善设备规格并评估在兽医环境中的临床效用。
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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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