Cisternal Contrast-Enhanced MRI Reveals Post-Stroke Glymphatic Impairment and Compensatory Metabolic Waste Clearance via Microglia/Macrophages.

IF 4.3 2区医学 Q1 CLINICAL NEUROLOGY

Translational Stroke Research Pub Date : 2025-09-10 DOI:10.1007/s12975-025-01373-x

Chengfeng Sun, Chanchan Li, Luyi Lin, Lekang Yin, Jiaojiao Li, Yan Ren, Weijun Tang, Yanmei Yang

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Abstract

Recent studies have shown that the glymphatic system plays a crucial role in driving hyperacute edema after ischemic stroke. This has sparked interest in understanding how this system changes in later phases of ischemic stroke. In this study, we utilized cisternal contrast-enhanced magnetic resonance imaging (CE-MRI) and immunofluorescence staining to investigate glymphatic system alterations at subacute and chronic phases of ischemic stroke. Middle cerebral artery occlusion (MCAO) for 90 min in Sprague-Dawley rats was used to mimic ischemic stroke. A total of 20 rats were randomly divided into four groups: sham group, MCAO 1-week group, MCAO 2-week group, and MCAO 2-month group. Our results showed the glymphatic system was spatially and temporally heterogeneously impaired in the peri-infarct area at subacute phase, even lasting for chronic phase. Specially, we found retention of contrast after cisternal CE-MRI in the infarct core and peri-infarct area at subacute phase of ischemic stroke, which corresponded to the distribution of microglia/macrophages. Our results indicated that ischemic stroke contributed to long-term glymphatic impairment and waste retention, and cisternal CE-MRI delayed enhancement could reflect the retention of waste and activation of microglia/macrophages in this process. These findings suggest cisternal CE-MRI might be a useful tool for investigating the interaction between the glymphatic system and microglia/macrophages in waste clearance and neuroinflammation after brain insult.

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脑池增强MRI显示脑卒中后淋巴系统损伤和代偿性代谢废物通过小胶质细胞/巨噬细胞清除。

最近的研究表明，淋巴系统在缺血性脑卒中后的超急性水肿中起着至关重要的作用。这激发了人们对了解该系统在缺血性中风后期如何变化的兴趣。在这项研究中，我们利用池对比增强磁共振成像（CE-MRI）和免疫荧光染色来研究缺血性卒中亚急性期和慢性期淋巴系统的改变。采用脑中动脉闭塞（MCAO） 90 min模拟脑缺血。将20只大鼠随机分为4组：假手术组、MCAO 1周组、MCAO 2周组、MCAO 2个月组。我们的研究结果表明，在亚急性期，甚至持续到慢性期，梗死周围区域的淋巴系统在空间和时间上都存在异质性。特别地，我们在缺血性卒中亚急性期发现脑池CE-MRI后梗死核心和梗死周围区域的造影剂保留，这与小胶质细胞/巨噬细胞的分布相对应。我们的研究结果表明，缺血性卒中导致了长期的淋巴细胞损伤和废物滞留，而池CE-MRI延迟增强可以反映废物滞留和小胶质细胞/巨噬细胞的激活。这些发现表明，池CE-MRI可能是研究脑损伤后淋巴系统和小胶质细胞/巨噬细胞在废物清除和神经炎症中的相互作用的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Stroke Research CLINICAL NEUROLOGY-NEUROSCIENCES

CiteScore

13.80

自引率

4.30%

发文量

130

审稿时长

6-12 weeks

期刊介绍： Translational Stroke Research covers basic, translational, and clinical studies. The Journal emphasizes novel approaches to help both to understand clinical phenomenon through basic science tools, and to translate basic science discoveries into the development of new strategies for the prevention, assessment, treatment, and enhancement of central nervous system repair after stroke and other forms of neurotrauma. Translational Stroke Research focuses on translational research and is relevant to both basic scientists and physicians, including but not restricted to neuroscientists, vascular biologists, neurologists, neuroimagers, and neurosurgeons.