Eco-friendly microencapsulation of Lacticaseibacillus paracasei using Ficus pumila seed extract: A novel plant-based delivery system enhancing probiotic stability and gastrointestinal tolerance.

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-07-01 Epub Date: 2025-07-27 DOI:10.14202/vetworld.2025.2039-2050
Watcharapong Mitsuwan, Chonticha Romyasamit, Rungruedee Kimseng, Tanakamol Mahawan, Sasi Vimon
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引用次数: 0

Abstract

Background and aim: Probiotic viability remains a critical challenge during gastrointestinal (GI) transit, storage, and feed processing. Conventional encapsulation materials often fail under acidic and thermal stress. This study aimed to develop and characterize a novel, eco-friendly microencapsulation system using Ficus pumila (FP) seed extract as a natural encapsulating matrix for Lacticaseibacillus paracasei (LP) WU2502, enhancing its functional resilience and storage stability.

Materials and methods: Microcapsules containing LP and FP were formulated through ionic gelation using calcium chloride. Physicochemical properties were assessed using scanning electron microscopy and Fourier-transform infrared (FTIR). Functional evaluations included encapsulation efficiency (EE), swelling kinetics, controlled release in simulated gastric and intestinal fluids, stress tolerance (acid, bile, enzymes, thermal), and viability during 60-day storage at 4°C and 25°C.

Results: The LP/FP microcapsules demonstrated high EE (80.5%) and spherical morphology (~200μm). FTIR confirmed the presence of ionic and hydrogen bonding in the matrix. The system exhibited pH-responsive swelling and controlled release, reaching 89.17% cumulative release in intestinal fluid. Encapsulated cells showed significantly improved tolerance to acidic pH, bile salts, digestive enzymes, and heat compared to free cells (p < 0.05). After 60 days, the viability of encapsulated cells remained above 60% at both storage temperatures, while free cell viability dropped by over 85%.

Conclusion: FP seed extract offers a biodegradable, plant-derived alternative for probiotic encapsulation. The developed LP/FP system effectively enhances probiotic survival under GI and thermal stress and during extended storage. These results support its application as a sustainable delivery platform for animal feed and functional food formulations.

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用无花果种子提取物微胶囊化副casasei乳酸菌:一种新的基于植物的传递系统,增强益生菌的稳定性和胃肠道耐受性。
背景和目的:益生菌的生存能力在胃肠道(GI)运输、储存和饲料加工过程中仍然是一个关键的挑战。传统的封装材料在酸性和热应力下往往失效。本研究旨在以无花果(Ficus pumila, FP)种子提取物为天然包封基质,开发并表征一种新型的生态友好型微胶囊体系,以提高副卡萨乳酸菌(Lacticaseibacillus paracasei, LP) WU2502的功能弹性和储存稳定性。材料与方法:采用氯化钙离子凝胶法制备LP和FP微胶囊。利用扫描电子显微镜和傅里叶变换红外(FTIR)对其理化性质进行了评估。功能评估包括包封效率(EE)、肿胀动力学、在模拟胃液和肠液中的控释、应激耐受性(酸、胆汁、酶、热)以及在4°C和25°C条件下60天的生存能力。结果:LP/FP微胶囊具有高EE(80.5%)和球形形貌(~200μm)。FTIR证实基体中存在离子键和氢键。该系统具有ph响应性肿胀和控释,在肠液中累积释放达到89.17%。与游离细胞相比,包被细胞对酸性pH、胆盐、消化酶和热的耐受性显著提高(p < 0.05)。60天后,在两种储存温度下,包被细胞的活力都保持在60%以上,而自由细胞的活力下降了85%以上。结论:FP种子提取物为益生菌包封提供了一种可生物降解的植物源性替代品。开发的LP/FP系统可有效提高益生菌在GI和热胁迫下以及延长贮藏期的存活率。这些结果支持其作为动物饲料和功能性食品配方的可持续输送平台的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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