Min-Gyu Kim, Seong-Keun Lim, Jun-Woo Choi, Jaeduk Goh, Chang Soo Lee, Hye Yeon Mun, Seung-Yeol Lee, Hee-Young Jung
{"title":"Identification and Characterization of Five Previously Unrecorded <i>Penicillium</i> Species of Subgenus <i>Aspergilloides</i> Isolated in Korea.","authors":"Min-Gyu Kim, Seong-Keun Lim, Jun-Woo Choi, Jaeduk Goh, Chang Soo Lee, Hye Yeon Mun, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2025.2550814","DOIUrl":null,"url":null,"abstract":"<p><p>In a survey of freshwater and near-freshwater soils in Chungbuk, Gyeonggi, Gangwon, Gyeongbuk, and Gyeongnam provinces in Korea, seven fungal strains were isolated and identified as members of the genus <i>Penicillium</i> based on the internal transcribed spacer (ITS) regions sequence analyses. Identification was performed through observing morphological characteristics and conducting phylogenetic analyses based on concatenated partial ITS, β-tubulin, calmodulin, and RNA polymerase II subunit gene sequences. The phylogenetic analyses revealed that strain NNIBRFG6577 was distinct from known <i>Penicillium</i> species, and based on morphological comparisons with the closest related species, <i>P. scruposum</i> CGMCC 3.25167<sup>T</sup> and <i>P. subasperum</i> CGMCC 3.25173<sup>T</sup>, it was proposed as a novel species. Strains NNIBRFG4823, NNIBRFG4610, and NNIBRFG4600 were closely related to <i>P. ellipsoideum</i> CGMCC 3.25156<sup>T</sup>, <i>P. skrjabinii</i> CBS 439.75<sup>T</sup>, and <i>P. yezoense</i> CBS 350.59<sup>T</sup>, respectively, while strains NNIBRFG1486, NNIBRFG48324, and NNIBRFG49919 clustered with <i>P. pancosmium</i> CBS 276.75<sup>T</sup>, and all strains showed morphological characteristics similar to those of their respective reference strains. These findings enrich Korea's fungus inventory, providing a basis for valuable future resource development.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 5","pages":"731-746"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12416006/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2025.2550814","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
In a survey of freshwater and near-freshwater soils in Chungbuk, Gyeonggi, Gangwon, Gyeongbuk, and Gyeongnam provinces in Korea, seven fungal strains were isolated and identified as members of the genus Penicillium based on the internal transcribed spacer (ITS) regions sequence analyses. Identification was performed through observing morphological characteristics and conducting phylogenetic analyses based on concatenated partial ITS, β-tubulin, calmodulin, and RNA polymerase II subunit gene sequences. The phylogenetic analyses revealed that strain NNIBRFG6577 was distinct from known Penicillium species, and based on morphological comparisons with the closest related species, P. scruposum CGMCC 3.25167T and P. subasperum CGMCC 3.25173T, it was proposed as a novel species. Strains NNIBRFG4823, NNIBRFG4610, and NNIBRFG4600 were closely related to P. ellipsoideum CGMCC 3.25156T, P. skrjabinii CBS 439.75T, and P. yezoense CBS 350.59T, respectively, while strains NNIBRFG1486, NNIBRFG48324, and NNIBRFG49919 clustered with P. pancosmium CBS 276.75T, and all strains showed morphological characteristics similar to those of their respective reference strains. These findings enrich Korea's fungus inventory, providing a basis for valuable future resource development.
期刊介绍:
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.