Cell wall invertase improves grain nutrition via regulating sugar and hormone metabolism gene expression in transgenic soybean.

IF 3.6 2区生物学 Q1 PLANT SCIENCES

Annals of botany Pub Date : 2025-09-10 DOI:10.1093/aob/mcaf221

Tingting Mao, Shengjie Zhuo, Zigui Geng, Shengnan Wang, Yaru Zhang, Feifei Gao, Yang Yu, Wenlong Geng, Chunyan Yu, Bei Li, Juan Zhang, Hongxia Zhang

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引用次数: 0

Abstract

Background and aims: Cell wall invertases have multiple roles in plant growth and development, yet their biological functions in seed oil production are still not understood.

Methods: In the present study, the Oryza sativa (rice) cell wall invertase gene OsGIF1 (GRAIN INCOMPLETE FILLING 1) was ectopically expressed in Glycine max (Soybean) and its functions in grain yield and seed nutrition was investigated.

Key results: We found that constitutive expression of OsGIF1 significantly improved biomass production, grain yield and seed nutrition in transgenic plants. The contents of protein and starch were significantly increased without dramatic alteration in the content of oil in the seeds of transgenic plants. In addition, the contents of histidine, tryptophan, asparagine, glutamine, palmitic acid and linoleic acid were increased, whereas the contents of oleic acid and linolenic acid were decreased. Further transcriptomic analyses in transgenic plants showed that genes associated with sugar and hormone metabolism were dramatically up-regulated.

Conclusions: Our findings offer direct evidence for the potential usage of this gene in the genetic breeding of oil crops with improved grain yield and nutrition.

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细胞壁转化酶通过调节转基因大豆糖和激素代谢基因的表达来改善籽粒营养。

背景与目的：细胞壁转化酶在植物生长发育中具有多种作用，但其在种子油脂生产中的生物学功能尚不清楚。方法：利用水稻细胞壁转化酶基因OsGIF1 （GRAIN INCOMPLETE FILLING 1）在大豆（Glycine max）中异位表达，研究其在籽粒产量和种子营养中的作用。主要结果：我们发现OsGIF1的组成型表达显著提高了转基因植物的生物量、籽粒产量和种子营养。转基因植物种子中蛋白质和淀粉含量显著增加，但油脂含量没有明显变化。组氨酸、色氨酸、天冬酰胺、谷氨酰胺、棕榈酸和亚油酸含量升高，油酸和亚麻酸含量降低。进一步的转录组学分析表明，与糖和激素代谢相关的基因显著上调。结论：本研究结果为该基因在油料作物遗传育种中的潜在应用提供了直接证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of botany 生物-植物科学

CiteScore

7.90

自引率

4.80%

发文量

138

审稿时长

3 months

期刊介绍： Annals of Botany is an international plant science journal publishing novel and rigorous research in all areas of plant science. It is published monthly in both electronic and printed forms with at least two extra issues each year that focus on a particular theme in plant biology. The Journal is managed by the Annals of Botany Company, a not-for-profit educational charity established to promote plant science worldwide. The Journal publishes original research papers, invited and submitted review articles, ''Research in Context'' expanding on original work, ''Botanical Briefings'' as short overviews of important topics, and ''Viewpoints'' giving opinions. All papers in each issue are summarized briefly in Content Snapshots , there are topical news items in the Plant Cuttings section and Book Reviews . A rigorous review process ensures that readers are exposed to genuine and novel advances across a wide spectrum of botanical knowledge. All papers aim to advance knowledge and make a difference to our understanding of plant science.