Revolutionizing meropenem detection: novel OVA-PtNP nanoenzymes for clinical and environmental safety.

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2025-09-10 DOI:10.1039/d5an00506j

Xiaoyun Lin, Wenli Lai, Limei Yang, Lina Wu, Yan Yuan, Xiaofen Hu, Liqing Lin

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Abstract

Purpose: The objective of this study is to develop a straightforward and expeditious clinical detection method for meropenem. This study aims to introduce an innovative nanoenzyme design, thereby broadening the application of platinum nanomaterials in biological detection. It seeks to facilitate the portable detection of meropenem using commercial software. Methods: Ovalbumin platinum nanoparticle units (OVA-PtNPs) were synthesized via a redox method, utilizing ovalbumin as a stabilizer for meropenem detection. The OVA-PtNPs induced a color change in TMB substrates, resulting in the formation of oxidized TMB with a strong ultraviolet absorption peak at 652 nm. Upon the addition of meropenem, the absorbance at this wavelength decreased, thereby enabling the detection of meropenem. Results: The experimental results confirmed that OVA-PtNPs were successfully prepared with a stable structure and significant oxidase-like activity, generating substantial superoxide anions (O₂˙^-). This method could detect meropenem in the range of 3.125-500 μg mL^-1, with a detection limit of 0.75 μg mL^-1. Furthermore, the meropenem concentrations in three groups of serum samples analyzed through spiked recovery experiments showed a relative standard deviation (RSD) of less than 3%. Conclusion: This research presents novel concepts for the design of nanoenzymes and the expanded application of platinum nanomaterials in biological detection.

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革新美罗培南检测：用于临床和环境安全的新型OVA-PtNP纳米酶。

目的：建立一种简便、快速的美罗培南临床检测方法。本研究旨在介绍一种创新的纳米酶设计，从而拓宽铂纳米材料在生物检测中的应用。它旨在促进使用商业软件对美罗培南进行便携式检测。方法：采用氧化还原法制备卵清蛋白铂纳米颗粒单元（OVA-PtNPs），以卵清蛋白为稳定剂检测美罗培南。OVA-PtNPs诱导TMB底物发生颜色变化，形成氧化的TMB，在652nm处有很强的紫外吸收峰。加入美罗培南后，该波长的吸光度降低，从而可以检测美罗培南。结果：实验结果证实成功制备了OVA-PtNPs，具有稳定的结构和显著的氧化酶样活性，产生大量的超氧阴离子（O2˙-）。方法检出范围为3.125 ~ 500 μg mL-1，检出限为0.75 μg mL-1。此外，通过加标回收率实验分析，三组血清样品中的美罗培南浓度的相对标准偏差（RSD）均小于3%。结论：本研究为纳米酶的设计和铂纳米材料在生物检测中的广泛应用提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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