An Effective Dual-Wavelength Fluorescent Sensor for ClO− Detection

Abstract

A novel dual-wavelength fluorescent probe targeting hypochlorite was successfully designed and synthesized, with its molecular structure unambiguously characterized by ¹H, ¹³C NMR spectroscopy and high-resolution mass spectrometry. This “off-on” type sensor demonstrates a distinctive dual-emission response at 494 and 599 nm upon hypochlorite activation. The proposed response mechanism, involving hypochlorite-induced specific structural modulation, not only achieves remarkable target specificity but also effectively suppresses background interference through its ratiometric detection modality. Importantly, both emission channels exhibit excellent linear correlations (R² > 0.995) with hypochlorite concentrations in the biologically relevant range of 50–180 μM, demonstrating its potential for quantitative analysis in complex matrices.