Cholesterol and highly unsaturated fatty acids: Exploring their synergistic roles in growth and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-09-10 DOI:10.1016/j.aqrep.2025.103091

Qiuran Yu , Song Wang , Erchao Li , Shubin Liu , Zhijuan Jiang , Xiaodan Wang , Jian G. Qin , Liqiao Chen

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引用次数: 0

Abstract

This study investigated the interactive effects of dietary cholesterol and highly unsaturated fatty acids (HUFAs) on the growth performance, molting dynamics, lipid metabolism, and nutrient signaling pathways in juvenile Chinese mitten crab (Eriocheir sinensis). A 3 × 2 factorial experiment was conducted over 8 weeks, manipulating three cholesterol levels (0 %, 0.06 %, 0.6 %) and two HUFAs levels (0 %, 0.4 %, EPA:DHA 1:3). Results demonstrated a significant synergistic interaction between dietary cholesterol and HUFAs. The combination of 0.06 % cholesterol and 0.4 % HUFAs yielded superior growth outcomes, including the highest final body weight, weight gain, specific growth rate, and lowest feed conversion ratio, surpassing the effects of individual supplementation. This optimal dietary combination also significantly increased whole-body crude protein and lipid content. Analysis of serum biochemical parameters revealed that cholesterol increased triacylglycerides, high-density lipoprotein cholesterol, and total cholesterol, while HUFAs decreased low-density lipoprotein cholesterol. Tissue lipid content and fatty acid profiles varied across molting stages. The combined diet enhanced lipid deposition in the hepatopancreas during the premolt stage. It also promoted the efficient utilization of lipids postmolt, particularly for EPA and DHA. Molecular analyses revealed a synergistic effect of the combined cholesterol and HUFAs supplementation, especially during the postmolt stage. This combination significantly upregulated key genes involved in lipid synthesis (srebp1, fas) in the hepatopancreas and muscle. Furthermore, it enhanced the expression of genes related to lipid transport (mttp, fatp, ldlr). Furthermore, the combined treatment significantly upregulated the expression of growth factor signaling pathway genes (mtorc1, s6k1, egfr, erk) in both the hepatopancreas and muscle. This upregulation likely facilitated rapid cell growth and tissue repair. These findings highlight that optimal dietary cholesterol and HUFAs interact synergistically to enhance growth performance and modulate lipid metabolism and growth signaling throughout the molting cycle in E. sinensis.

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胆固醇和高不饱和脂肪酸在中华绒螯蟹幼蟹生长和脂质代谢中的协同作用

研究了饲料胆固醇和高不饱和脂肪酸（HUFAs）对中华绒螯蟹（Eriocheir sinensis）幼蟹生长性能、换壳动态、脂质代谢和营养信号通路的交互作用。在8周的时间里进行了3 × 2因子实验，控制了3种胆固醇水平（0 %,0.06 %,0.6 %）和2种HUFAs水平（0 %,0.4 %,EPA:DHA 1:3）。结果表明，膳食胆固醇和HUFAs之间存在显著的协同作用。0.06 %胆固醇和0.4 % HUFAs的组合产生了优异的生长结果，包括最高的最终体重、增重、特定生长率和最低的饲料系数，超过了单独添加的效果。这一最佳膳食组合也显著提高了全鱼粗蛋白质和脂肪含量。血清生化指标分析显示，胆固醇升高甘油三酯、高密度脂蛋白胆固醇和总胆固醇，而HUFAs降低低密度脂蛋白胆固醇。组织脂质含量和脂肪酸分布在蜕皮阶段有所不同。联合饲粮增加了蜕皮前期肝胰腺的脂质沉积。它还促进了脱毛后脂质的有效利用，特别是EPA和DHA。分子分析显示，胆固醇和HUFAs的联合补充具有协同效应，特别是在脱毛后阶段。这种组合显著上调了肝胰腺和肌肉中参与脂质合成的关键基因（srebp1, fas）。此外，它还增强了脂质转运相关基因（mttp、fatp、ldlr）的表达。此外，联合治疗显著上调肝胰腺和肌肉中生长因子信号通路基因（mtorc1、s6k1、egfr、erk）的表达。这种上调可能促进了细胞的快速生长和组织修复。这些发现表明，在整个换毛周期中，最佳膳食胆固醇和HUFAs协同作用可提高中华绒螯蟹的生长性能，调节脂质代谢和生长信号。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.