Galectin-10 Silencing Reduces Eosinophilic Inflammation in Chronic Rhinosinusitis with Nasal Polyps by Inhibiting the p38/MAPK/NF-κB Pathway.

IF 0.9 4区医学 Q4 IMMUNOLOGY

Critical Reviews in Immunology Pub Date : 2025-01-01 DOI:10.1615/CritRevImmunol.2025060503

Chunhua Li, HongBing Liu

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引用次数: 0

Abstract

Galectin-10(Gal-10)/CLC(Charcot-Leyden crystal) has been discovered to be related to ECRSwNP characterized by high eosinophilic infiltration. We aimed to investigate the effects of Gal-10 on ECRSwNP. A total of 36 tissue samples were collected, including 11 ECRSwNP samples, 15 non-ECRSwNP samples, and 10 Control samples. Human eosinophils were divided into 3 groups: Control, siRNA-NC, and Gal-10 interference groups. Immunohistochemistry (IHC), Western blotting and quantitative real-time PCR (qRT-PCR) detected the expression of Gal-10 in the tissues samples and expression of p-p38 and p-p65 in human eosinophils. Enzyme-Linked Immunosorbent Assay (ELISA) was adopted to measure the expression levels of IL-4, IL-5, IL-8, MBP, ECP, and TNF-α in the human eosinophils. We found the expression of Gal-10 was significantly higher in ECRSwNP group (P < 0.05). The expression levels of IL-4, IL-5, IL-8, MBP and TNF-α in the Gal-10 interference group were lower (P < 0.05), but ECP had no statistical difference in human eosinophils. There showed the expression levels of p-p38 and p-p65 proteins in the Gal-10 interference group were lower (P < 0.05). The deletion of Gal-10 in eosinophils down-regulates the expression of cytokines and granule cationic proteins in ECRSwNP which may be caused by the p38MAPK/ NF-κB pathway.

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半乳糖凝集素-10沉默通过抑制p38/MAPK/NF-κB通路减少慢性鼻窦炎伴鼻息肉的嗜酸性粒细胞炎症。

半凝集素-10(Galectin-10, Gal-10)/CLC（Charcot-Leyden crystal, Charcot-Leyden crystal）已被发现与以高嗜酸性浸润为特征的ECRSwNP有关。我们的目的是研究Gal-10对ECRSwNP的影响。共收集组织样本36份，其中ECRSwNP样本11份，非ECRSwNP样本15份，对照样本10份。将人嗜酸性粒细胞分为3组：对照组、siRNA-NC组和Gal-10干扰组。免疫组织化学（IHC）、免疫印迹（Western blotting）和实时荧光定量PCR （qRT-PCR）检测组织样本中Gal-10的表达和人嗜酸性粒细胞中p-p38和p-p65的表达。采用酶联免疫吸附法（ELISA）检测人嗜酸性粒细胞中IL-4、IL-5、IL-8、MBP、ECP、TNF-α的表达水平。我们发现，在ECRSwNP组中Gal-10的表达显著升高（P < 0.05）。Gal-10干扰组IL-4、IL-5、IL-8、MBP、TNF-α表达水平降低（P < 0.05），但ECP对人嗜酸性粒细胞的表达差异无统计学意义。Gal-10干扰组P -p38、P -p65蛋白表达水平降低（P < 0.05）。嗜酸性粒细胞中Gal-10的缺失下调了ECRSwNP中细胞因子和颗粒阳离子蛋白的表达，这可能与p38MAPK/ NF-κB通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Immunology 医学-免疫学

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Immunology covers a broad spectrum of investigations at the genes, molecular, cellular, organ and system levels to reveal defense mechanisms against pathogens as well as protection against tumors and autoimmune diseases. The great advances in immunology in recent years make this field one of the most dynamic and rapidly growing in medical sciences. Critical ReviewsTM in Immunology (CRI) seeks to present a balanced overview of contemporary adaptive and innate immune responses related to autoimmunity, tumor, microbe, transplantation, neuroimmunology, immune regulation and immunotherapy from basic to translational aspects in health and disease. The articles that appear in CRI are mostly obtained by invitations to active investigators. But the journal will also consider proposals from the scientific community. Interested investigators should send their inquiries to the editor before submitting a manuscript.