Detection of pathogens and epidemiological characteristics of community-acquired pneumonia in children using metagenomic next-generation sequencing.

IF 1.2 4区医学 Q4 INFECTIOUS DISEASES

Journal of Infection in Developing Countries Pub Date : 2025-08-31 DOI:10.3855/jidc.20668

ShiQu Deng, YaLi Wu, PeiYun Hu, JianLiang Lin, Jingming Yao, Bin Wang

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引用次数: 0

Abstract

Introduction: Community-acquired pneumonia (CAP) is a common respiratory disease in children and a significant factor in child mortality.

Methodology: We aimed to investigate metagenomic next-generation sequencing (mNGS) technology to explore pathogens and epidemiological characteristics of pediatric CAP. We retrospectively analyzed mNGS detection and microbiological culture results of bronchoalveolar lavage fluid (BALF) and sputum samples from children with CAP.

Results: Mycoplasma pneumoniae was the predominant pathogen. Bacteria/fungi detection rates using mNGS in sputum and BALF were higher than those using microbiological culture (p < 0.05). Cytomegalovirus was the predominant pathogen in children aged 0-1; Mycoplasma pneumoniae was the predominant pathogen in those over 1. Haemophilus influenzae, Mycoplasma pneumoniae, human bocavirus 1, and Streptococcus pneumoniae were the predominant pathogens in spring, summer, autumn, and winter, respectively.

Conclusions: mNGS is superior to traditional microbiological culture for pediatric CAP potential pathogen detection. CAP pathogenic infection characteristics at different ages and seasons detected by mNGS will benefit clinical practitioners in the prevention and treatment of pediatric CAP in their local/regional areas.

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应用新一代宏基因组测序检测儿童社区获得性肺炎病原体及流行病学特征。

社区获得性肺炎（CAP）是儿童常见的呼吸道疾病，是儿童死亡的重要因素。方法：采用元基因组新一代测序（mNGS）技术探讨小儿CAP的病原菌及流行病学特征。回顾性分析小儿CAP的支气管肺泡灌洗液（BALF）及痰液mNGS检测及微生物培养结果。结果：支原体肺炎为优势病原菌。痰液和BALF中mNGS的细菌/真菌检出率高于微生物培养（p < 0.05）。0 ~ 1岁儿童以巨细胞病毒为主；1岁以上人群以肺炎支原体为主。春季、夏季、秋季和冬季分别以流感嗜血杆菌、肺炎支原体、人博卡病毒1型和肺炎链球菌为主。结论：mNGS检测儿科CAP潜在病原菌优于传统微生物培养。mNGS检测不同年龄、不同季节的小儿CAP病原感染特征，有助于临床工作者在本地区开展小儿CAP的防治工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection in Developing Countries INFECTIOUS DISEASES-

CiteScore

3.70

自引率

5.30%

发文量

239

审稿时长

4-8 weeks

期刊介绍： The Journal of Infection in Developing Countries (JIDC) is an international journal, intended for the publication of scientific articles from Developing Countries by scientists from Developing Countries. JIDC is an independent, on-line publication with an international editorial board. JIDC is open access with no cost to view or download articles and reasonable cost for publication of research artcles, making JIDC easily availiable to scientists from resource restricted regions.