The Function of Hsa_circ_0001862 Regulating miR-23a-IRF1/PPP2R5E Axis to Oral Squamous Cell Carcinoma Development.

Abstract

Backgrounds: Head and neck cancer is among the top ten cancers worldwide, with most lesions in the oral cavity. Oral squamous cell carcinoma accounts for more than 90% of all oral malignancies and is a significant public health concern. Circular RNA and micro RNA, as non-coding RNA, plays an important role in the development of tumor transmission.

Aims: This study attempted to explore the pathogenesis of oral squamous cell carcinoma from the perspective of non-coding RNA.

Materials & methods: We found that miR-23a was abnormally elevated in oral squamous cell carcinoma tissues. Taking this as an entry point, we searched for the circRNA and downstream target genes of miR-23a's upstream function through bioinformatics prediction. We determined the direct targeting relationship between miR-23a and hsa_circ_0001862, as well as the downstream target genes IRF1 and PPP2R5E, by dual luciferase reporter experiments. We used a series of cell phenotype experiments, such as CCK8, colony formation, scratch and trans-well assays, to verify the influence of each of the above genes on the tongue squamous cell line Tca-8113.

Results: Hsa_circ_0001862 regulates the expression level of miR-23a through sponging. In the occurrence and development of tongue squamous cell carcinoma, the expression level of miR-23a is abnormally increased, which can promote the occurrence and development of tongue squamous cell carcinoma by down-regulating the expression level of its target genes IRF1 and PPP2R5E.

Discussion: The identification of this signaling pathway provides a practical basis for early detection, early diagnosis and early treatment of oral squamous cell carcinoma.

Conclusion: The regulatory mechanism of hsa_circ_0001862-miR-23a-IRF1/PPP2R5E axis on the OSCC and the function of downstream target genes that play specific roles.